Eastwood, T., Baker, Karen, Brooker, Holly, Frank, Stefanie, Mulvihill, Daniel P. (2017) An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affection alpha-synuclein oligomerisation. FEBS letters, 591 (6). pp. 833-841. ISSN 0014-5793. (doi:10.1002/1873-3468.12597) (KAR id:60373)
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| Official URL http://dx.doi.org/10.1002/1873-3468.12597 |
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Abstract
Amino terminal acetylation is a ubiquitous protein modification affecting the majority of eukaryote proteins to regulate stability and function. We describe an optimised recombinant expression system for rapid production of aminoterminal-acetylated proteins within bacteria. We go on to describe the
dependent oligomerisation. These new tools and protocols will allow researchers to enhance routine recombinant protein production and identify
new molecules for use in research and clinical applications.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1002/1873-3468.12597 |
| Subjects: | Q Science |
| Divisions: | Divisions > Division of Natural Sciences > School of Biosciences |
| Depositing User: | Susan Davies |
| Date Deposited: | 14 Feb 2017 16:13 UTC |
| Last Modified: | 06 Feb 2020 04:15 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/60373 (The current URI for this page, for reference purposes) |
| Mulvihill, Daniel P.: |  https://orcid.org/0000-0003-2502-5274 |
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