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Investigating the effects of novel AKT inhibitors on the insulin/IGF-1 like signalling pathway in C. elegans

Doyle, Kevin (2016) Investigating the effects of novel AKT inhibitors on the insulin/IGF-1 like signalling pathway in C. elegans. Master of Research (MRes) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

Caenorhabditis elegans, a free-living nematode, has developed into an exciting model for the study

can be manipulated to extend its lifespan and healthspan. The best characterised of these pathways is the

the pathway. Inhibition of IIS results in the translocation of DAF-16 into the nucleus, allowing it to activate

The aim of this study was to pharmacologically inhibit the IIS pathway using the ATP-competitive AKT

ROCK inhibitor GSK-269962. A C. elegans transgene strain possessing a DAF-16::GFP reporter in order to

compounds and scored for the nuclear localisation of DAF-16. A bus-8 strain of C. elegans with increased

and GSK-269962 could be having an effect on DAF-16 nuclear localisation. Further studies are needed in

of a stress response to high concentrations of the drug.

Item Type: Thesis (Master of Research (MRes))
Thesis advisor: Garrett, Michelle
Thesis advisor: Tullet, Jennifer M.A.
Uncontrolled keywords: Cell Biology, C. elegans, Ageing, AKT-inhibitors, DAF-16
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Users 1 not found.
Date Deposited: 20 Jan 2017 16:00 UTC
Last Modified: 13 Mar 2020 04:08 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/59976 (The current URI for this page, for reference purposes)
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