Raap, U, Gehring, M, Kleiner, S, Rüdrich, U, Eiz-Vesper, B, Haas, Helmut, Kapp, A, Gibbs, Bernhard F (2017) Human Basophils are Differentially Activated by and are a Source of IL-31. Clinical and Experimental Allergy, 47 (4). pp. 499-508. ISSN 0954-7894. E-ISSN 1365-2222. (doi:10.1111/cea.12875) (KAR id:59776)
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Official URL: http://dx.doi.org/10.1111/cea.12875 |
Abstract
BackgroundBasophils are important effector cells involved in the pathogenesis of inflammatory skin diseases including chronic urticaria which is associated by increased IL-31 serum levels. So far the effects of IL-31 on human basophils are unknown.ObjectiveTo analyse the functional role of IL-31 in basophil biology.MethodsIL-31 expression was evaluated in skin samples derived from chronic spontaneous urticaria patients. Oncostatin M receptor (OSMR), IL-31 receptor A (RA) and IL-31 protein expressions were analysed on human basophils from healthy donors. Basophil responses to IL-31 were assessed for chemotaxis, externalization of CD63 and CD203c as well as the release of histamine, IL-4 and IL-13.ResultsIL-31RA and OSMR were expressed on human basophils. IL-31 was strongly expressed in the skin of patients with chronic spontaneous urticaria and was released from isolated basophils following either anti-IgE, IL-3 or fMLP stimulation. IL-31 induced chemotaxis and the release of IL-4 and IL-13 which was specifically inhibited by anti-IL-31RA and anti-OSMR. Conversely, IL-31 had no effect on CD63 and CD203c externalization or histamine release.Conclusions and Clinical RelevanceHuman basophils are a source of –and are activated by – IL-31 with the release of pro-inflammatory cytokines and the induction of chemotaxis indicating an important novel function of IL-31 in basophil biology.
Item Type: | Article |
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DOI/Identification number: | 10.1111/cea.12875 |
Uncontrolled keywords: | Basophils, Il-31, allergy |
Subjects: |
Q Science > QR Microbiology > QR180 Immunology R Medicine > RL Dermatology |
Divisions: | Divisions > Division of Natural Sciences > Medway School of Pharmacy |
Depositing User: | Bernhard F. Gibbs |
Date Deposited: | 04 Jan 2017 14:12 UTC |
Last Modified: | 04 Mar 2024 15:22 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/59776 (The current URI for this page, for reference purposes) |
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