Human Basophils are Differentially Activated by and are a Source of IL-31

Raap, U and Gehring, M and Kleiner, S and Rüdrich, U and Eiz-Vesper, B and Haas, Helmut and Kapp, A and Gibbs, Bernhard F (2016) Human Basophils are Differentially Activated by and are a Source of IL-31. Clinical and Experimental Allergy, . ISSN 0954-7894. (In press) (doi:https://doi.org/10.1111/cea.12875/full) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

(Unedited version) Background: Basophils are important effector cells involved in the pathogenesis of inflammatory skin diseases including chronic urticaria which is associated by increased IL-31 serum levels. So far the effects of IL-31 on human basophils are unknown. Objective: To analyse the functional role of IL-31 in basophil biology. Methods: IL-31 expression was evaluated in skin samples derived from chronic spontaneous urticaria patients. Oncostatin M receptor (OSMR), IL-31 receptor A (RA) and IL-31 protein expressions were analysed on human basophils from healthy donors. Basophil responses to IL-31 were assessed for chemotaxis, externalization of CD63 and CD203c as well as the release of histamine, IL-4 and IL-13. Results: IL-31RA and OSMR were expressed on human basophils. IL-31 was strongly expressed in the skin of patients with chronic spontaneous urticaria and was released from isolated basophils following either anti-IgE, IL-3 or fMLP stimulation. IL-31 induced chemotaxis and the release of IL-4 and IL-13 which was specifically inhibited by anti-IL-31RA and anti-OSMR. Conversely, IL-31 had no effect on CD63 and CD203c externalization or histamine release. Conclusions and clinical relevance: Human basophils are a source of -and are activated by- IL-31 with the release of pro-inflammatory cytokines and the induction of chemotaxis indicating an important novel function of IL-31 in basophil biology.

Item Type: Article
Uncontrolled keywords: Basophils, Il-31, allergy
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > RL Dermatology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Bernhard F. Gibbs
Date Deposited: 04 Jan 2017 14:12 UTC
Last Modified: 16 Feb 2017 11:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/59776 (The current URI for this page, for reference purposes)
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