Gangl, Doris (2016) Biotechnological Exploitation of Chlamydomonas reinhardtii. Doctor of Philosophy (PhD) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:56648)
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Abstract
Microalgae have become increasingly important in the biotech sector and are currently exploited for their natural products. In recent years efforts have also been directed towards establishing them as production platforms for recombinant proteins. The aim of this thesis is to investigate the feasibility of Chlamydomonas reinhardtii as a production platform for highvalue products expressed in the chloroplast of the alga. A cytochrome P450 was introduced
the chloroplast to date. The two transgenic strains expressing CYP79A1 and TPS4 were investigated for their ability to withstand industrial growth conditions. The cell wall deficient strains were successfully cultivated in 100 L photobioreactors using a mixotrophic growth regime. They reached dry weights of 0.3 g/L and the expression of CYP79A1 and TPS4 was
could be an attractive platform for recombinant protein production. Two enzymes with
cell wall deficient strains were successfully cultivated on a semi-industrial scale.
Item Type: | Thesis (Doctor of Philosophy (PhD)) |
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Thesis advisor: | Robinson, Colin |
Uncontrolled keywords: | Microalgae Chlamydomonas Chloroplast transformation Cytochrome P450 CYP79A1 Diterpene synthase cis-abienol synthase TPS4 Cell wall-deficient Pilot scale |
Subjects: | Q Science > QH Natural history > QH541 Ecology |
Divisions: | Divisions > Division of Natural Sciences > School of Biosciences |
Depositing User: | Users 1 not found. |
Date Deposited: | 27 Jul 2016 09:41 UTC |
Last Modified: | 23 Jan 2020 04:11 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/56648 (The current URI for this page, for reference purposes) |
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