Zinc carnosine works with bovine colostrum in truncating heavy exercise–induced increase in gut permeability in healthy volunteers

Davison, Glen and Marchbank, Tania and March, Daniel Scott and Thatcher, Rhys and Playford, Raymond J (2016) Zinc carnosine works with bovine colostrum in truncating heavy exercise–induced increase in gut permeability in healthy volunteers. American Journal of Clinical Nutrition, 104 (2). ISSN 0002-9165. (doi:https://doi.org/10.3945/?ajcn.116.134403) (Full text available)

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Abstract

Background: Heavy exercise causes gut symptoms and, in extreme cases, heat stroke that is due to the increased intestinal permeability of luminal toxins. Objective: We examined whether zinc carnosine (ZnC), a health-food product taken alone or in combination with bovine colostrum (a natural source of growth factors), would moderate such effects. Design: Eight volunteers completed a 4-arm, double-blind, placebo-controlled crossover protocol (14 d of placebo, ZnC, colostrum, or ZnC plus colostrum) before undertaking standardized exercise 2 and 14 d after the start of treatment. Changes in epithelial resistance, apoptosis signaling molecules, and tight junction (TJ) protein phosphorylation in response to a 2°C rise in body temperature were determined with the use of Caco-2 and HT29 intestinal cells. Results: Body temperature increased 2°C, and gut permeability (5-h urinary lactulose:rhamnose ratios) increased 3-fold after exercise (from 0.32 ± 0.016 baseline to 1.0 ± 0.017 at 14 d; P < 0.01). ZnC or colostrum truncated the rise by 70% after 14 d of treatment. The combination treatment gave an additional benefit, and truncated exercise induced increase at 2 d (30% reduction; P < 0.01). A 2°C temperature rise in in vitro studies caused the doubling of apoptosis and reduced epithelial resistance 3–4-fold. ZnC or colostrum truncated these effects (35–50%) with the greatest response seen with the combination treatment (all P < 0.01). Mechanisms of action included increasing heat shock protein 70 and truncating temperature-induced changes in B cell leukemia/lymphoma-2 associated X protein ? and B cell lymphoma 2. ZnC also increased total occludin and reduced phosphorylated tyrosine claudin, phosphorylated tyrosine occludin, and phosphorylated serine occludin, thereby enhancing the TJ formation and stabilization. Conclusion: ZnC, taken alone or with colostrum, increased epithelial resistance and the TJ structure and may have value for athletes and in the prevention of heat stroke in military personnel. This trial was registered at www.isrctn.com as ISRCTN51159138.

Item Type: Article
Uncontrolled keywords: leaky gut, intestinal permeability, gut damage, health, clinical trial, gut growth, injury, nutriceutical, repair
Subjects: G Geography. Anthropology. Recreation > GV Recreation Leisure > Sports sciences
Q Science > QP Physiology (Living systems)
R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC1200 Sports Medicine
R Medicine > RC Internal medicine > RC1235 Physiology of sports
U Military Science > U Military Science (General)
Divisions: Faculties > Sciences > School of Sport and Exercise Sciences
Depositing User: Glen Davison
Date Deposited: 21 Jul 2016 15:55 UTC
Last Modified: 29 Jun 2017 23:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/56573 (The current URI for this page, for reference purposes)
Davison, Glen: https://orcid.org/0000-0003-4340-0074
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