The mechanical response of talin

Yao, Mingxi and Goult, Benjamin T and Klapholz, Benjamin and Hu, Xian and Toseland, Christopher P and Guo, Yingjian and Cong, Peiwen and Sheetz, Michael P and Yan, Jie (2016) The mechanical response of talin. Nature Communications, 7 (11966). pp. 1-11. ISSN 2041-1723. (doi:https://doi.org/10.1038/ncomms11966) (Full text available)

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Abstract

Talin, a force-bearing cytoplasmic adapter essential for integrin-mediated cell adhesion, links the actin cytoskeleton to integrin-based cell–extracellular matrix adhesions at the plasma membrane. Its C-terminal rod domain, which contains 13 helical bundles, plays important roles in mechanosensing during cell adhesion and spreading. However, how the structural stability and transition kinetics of the 13 helical bundles of talin are utilized in the diverse talin-dependent mechanosensing processes remains poorly understood. Here we report the force-dependent unfolding and refolding kinetics of all talin rod domains. Using experimentally determined kinetics parameters, we determined the dynamics of force fluctuation during stretching of talin under physiologically relevant pulling speeds and experimentally measured extension fluctuation trajectories. Our results reveal that force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effective force buffer that sets a physiological force range of only a few pNs in the talin-mediated force transmission pathway.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 07 Jul 2016 11:18 UTC
Last Modified: 14 Jul 2016 13:52 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/56237 (The current URI for this page, for reference purposes)
Goult, Benjamin T: https://orcid.org/0000-0002-3438-2807
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