Modestobacter caceresii sp. nov., novel actinobacteria with an insight into their adaptive mechanisms for survival in extreme hyper-arid Atacama Desert soils

Busarakam, Kanungnid and Bull, Alan T. and Trujillo, Martha E. and Riesco, Raul and Sangal, Vartul and van Wezel, Gilles P. and Goodfellow, Michael (2016) Modestobacter caceresii sp. nov., novel actinobacteria with an insight into their adaptive mechanisms for survival in extreme hyper-arid Atacama Desert soils. Systematic and Applied Mircobiology, 39 (4). pp. 243-251. ISSN 0723-2020. (doi:https://doi.org/10.1016/j.syapm.2016.03.007) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://doi.org/10.1016/j.syapm.2016.03.007

Abstract

A polyphasic study was designed to determine the taxonomic provenance of three Modestobacter strains isolated from an extreme hyper-arid Atacama Desert soil. The strains, isolates KNN 45-1a, KNN 45-2bT and KNN 45-3b, were shown to have chemotaxonomic and morphological properties in line with their classification in the genus Modestobacter. The isolates had identical 16S rRNA gene sequences and formed a branch in the Modestobacter gene tree that was most closely related to the type strain of Modestobacter marinus (99.6% similarity). All three isolates were distinguished readily from Modestobacter type strains by a broad range of phenotypic properties, by qualitative and quantitative differences in fatty acid profiles and by BOX fingerprint patterns. The whole genome sequence of isolate KNN 45-2bT showed 89.3% average nucleotide identity, 90.1% (SD: 10.97%) average amino acid identity and a digital DNA–DNA hybridization value of 42.4 ± 3.1 against the genome sequence of M. marinus DSM 45201T, values consistent with its assignment to a separate species. On the basis of all of these data, it is proposed that the isolates be assigned to the genus Modestobacter as Modestobacter caceresii sp. nov. with isolate KNN 45-2bT (CECT 9023T = DSM 101691T) as the type strain. Analysis of the whole-genome sequence of M. caceresii KNN 45-2bT, with 4683 open reading frames and a genome size of ∽4.96 Mb, revealed the presence of genes and gene-clusters that encode for properties relevant to its adaptability to harsh environmental conditions prevalent in extreme hyper arid Atacama Desert soils.

Item Type: Article
Uncontrolled keywords: Modestobacter caceserii; Polyphasic taxonomy; Whole genome sequence; Hyper-arid Atacama Desert soil; Average nucleotide identity; Average amino acid identity
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 10 Jun 2016 09:38 UTC
Last Modified: 26 Sep 2016 11:29 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/55865 (The current URI for this page, for reference purposes)
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