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Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma

Smith, Jennifer R., Moreno, Lucas, Heaton, Simon P., Chesler, Louis, Pearson, Andrew D.J., Garrett, Michelle D. (2016) Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma. Molecular Oncology, 10 (4). pp. 538-552. ISSN 1574-7891. (doi:10.1016/j.molonc.2015.11.005) (KAR id:55391)

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http://doi.org/10.1016/j.molonc.2015.11.005

Abstract

There is an urgent need for improved therapies for children with high-risk neuroblastoma

associated with aggressive neuroblastoma and drugs targeting PI3K/AKT/mTOR, to activate

validate pharmacodynamic (PD) biomarkers to evaluate both proof of mechanism and proof

Wehave addressed the issue of limited access to tumor biopsies for quantitative detection

(FACS) method to purify CD45?/GD2+/CD56+ neuroblastoma cells from bone marrow. We

cells, providing the potential to demonstrate proof of concept for drugs that inhibit

detection of three biomarkers for AKT pathway activity (phosphorylated and total AKT,

ournewapproachto neuroblastomacell isolation for protein detection and suite ofPD assays

PD biomarkers in this pediatric patient population. These will be ideal tools to support

in upcoming clinical trials in neuroblastoma.

Item Type: Article
DOI/Identification number: 10.1016/j.molonc.2015.11.005
Uncontrolled keywords: Neuroblastoma; MYCN; AKT; Pharmacodynamic; Biomarker
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Michelle Garrett
Date Deposited: 11 May 2016 15:02 UTC
Last Modified: 27 Jan 2021 15:08 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/55391 (The current URI for this page, for reference purposes)
Garrett, Michelle D.: https://orcid.org/0000-0002-3939-1673
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