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Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity

Kumar, Abhishek, Ouyang, Mingxing, Van den Vries, Koen, McGhee, Ewan James, Tanaka, Keiichiro, Anderson, Marie D., Groisman, Alexander, Goult, Benjamin T., Anderson, Kurt I., Schwartz, Martin A. and others. (2016) Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. Journal of Cell Biology, 213 (3). pp. 371-383. ISSN 0021-9525. (doi:10.1083/jcb.201510012) (KAR id:55338)

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Integrin-dependent adhesions are mechanosensitive structures in which talin mediates a linkage to actin laments either directly or indirectly by recruiting vinculin. Here, we report the development and validation of a talin tension sensor. We nd that talin in focal adhesions is under tension, which is higher in peripheral than central adhesions. Tension on talin is increased by vinculin and depends mainly on actin-binding site 2 (ABS2) within the middle of the rod domain, rather than ABS3 at the far C terminus. Unlike vinculin, talin is under lower tension on soft substrates. The difference between central and peripheral adhesions requires ABS3 but not vinculin or ABS2. However, differential stiffness sensing by talin requires ABS2 but not vinculin or ABS3. These results indicate that central versus peripheral adhesions must be orga- nized and regulated differently, and that ABS2 and ABS3 have distinct functions in spatial variations and stiffness sens- ing. Overall, these results shed new light on talin function and constrain models for cellular mechanosensing.

Item Type: Article
DOI/Identification number: 10.1083/jcb.201510012
Uncontrolled keywords: adhesion; biophysics; cytoskeleton
Subjects: Q Science > QH Natural history > QH581.2 Cell Biology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 10 May 2016 05:50 UTC
Last Modified: 27 Jan 2021 12:31 UTC
Resource URI: (The current URI for this page, for reference purposes)
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