The Candida Albicans Gene Encoding the Cytoplasmic Leucyl-tRNA Synthetase: Implications for the Evolution of CUG Codon Reassignment

O'Sullivan, Justin M. and Mihr, Marian J. and Santos, Manuel and Tuite, Mick F. (2001) The Candida Albicans Gene Encoding the Cytoplasmic Leucyl-tRNA Synthetase: Implications for the Evolution of CUG Codon Reassignment. Gene, 275 (1). pp. 133-140. ISSN 0378-1119. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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In a number of Candida species the 'universal' leucine codon CUG is decoded as serine. To help understand the evolution of such a codon reassignment we have analyzed the Candida albicans leucyl-tRNA synthetase (CaLeuRS) gene (CaCDC60). The predicted CaLeuRS sequence shows a significant level of amino acid identity to LeuRS from other organisms. A mitochondrial LeuRS (ScNAM2) homologue, which shared low identity with the CaLeuRS, was also identified in C. albicans. Antigenically-related LeuRSs were identified in a range of Candida species decoding the CUG codon as both serine and leucine, using an antibody raised against the N-terminal 15 amino acids of the CaLeuRS. Complementation experiments demonstrated that the CaLeuRS was able to functionally complement a Saccharomyces cerevisiae cdc60:kanMX null mutation. We conclude that there is no alteration in tRNA recognition and aminoacylation by the C. albicans LeuRS, which argues against it having a role in codon reassignment. The nucleotide sequences of the CaCDC60 and CaNAM2 genes were deposited at GenBank under Accession numbers AF293346 and AF352020, respectively.

Item Type: Article
Additional information: 0378-1119 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Amino Acid Sequence Candida albicans/enzymology/*genetics Cloning, Molecular Codon/genetics Cytoplasm/enzymology DNA, Fungal/chemistry/genetics Evolution, Molecular Genes, Fungal/genetics Genetic Code/genetics Genetic Complementation Test Leucine-tRNA Ligase/*genetics Molecular Sequence Data Saccharomyces cerevisiae/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Mick Tuite
Date Deposited: 28 Oct 2008 18:39
Last Modified: 10 Jun 2014 11:20
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