15N NMR relaxation and structural elucidation of peptides in the presence and absence of trifluoroethanol illuminates the critical molecular nature of integrin ?v?6 ligand specificity

Howard, MJ (2012) 15N NMR relaxation and structural elucidation of peptides in the presence and absence of trifluoroethanol illuminates the critical molecular nature of integrin ?v?6 ligand specificity. RSC Advances, 2 . pp. 11019-11028. E-ISSN 2046-2069. (doi:10.1039/C2RA21655H)

Abstract

Integrin ?v?6 is an important emerging target for both imaging and therapy of cancer that requires specific ligands based on Arg-Gly-Asp (RGD) peptides. There remains little correlation between integrin-RGD ligand specificity despite studies suggesting an RGD-turn-helix ligand motif is required. Here, we describe the application of 15N NMR relaxation analyses and structure determination of ?v?6 peptide ligands in the presence and absence of trifluoroethanol (TFE) to identify their critical molecular nature that influences specificity, interaction and function. Two linear peptides; one known to demonstrate ?v?6 specificity (FMDV2) and the other based on a natural RGD ligand (LAP2), were compared to two additional peptides based on FMDV2 but cyclised in different positions using a disulphide bond (DBD1 and DBD2). The cyclic adaptation in DBD1 produces a significant alteration in backbone dynamic properties when compared to FMDV2; a potential driver for the loss in ?v?6 specificity by DBD1. The importance of ligand dynamics are highlighted through a comprehensive reduced spectral density and ModelFree analysis of peptide 15N NMR relaxation data and suggest ?v?6 specificity requires the formation of a structurally rigid helix preceded by a RGD motif exhibiting slow internal motion. Additional observations include the effect of TFE/water viscosity on global NMR dynamics and the advantages of using spectral density NMR relaxation data to estimate correlation times and motional time regimes for peptides in solution.

Item Type:	Article
DOI/Identification number:	10.1039/C2RA21655H
Subjects:	Q Science > QD Chemistry > QD431 Organic Chemistry- Biochemistry- Proteins, peptides, amino acids
Divisions:	Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User:	M.J. Howard
Date Deposited:	23 Mar 2016 17:03 UTC
Last Modified:	29 May 2019 17:07 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/54613 (The current URI for this page, for reference purposes)