The [PSI+] prion of yeast: a problem of inheritance

Tuite, Mick F. and Cox, Brian S. (2006) The [PSI+] prion of yeast: a problem of inheritance. Methods, 39 (1). pp. 9-22. ISSN 1046-2023. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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The [PSI(+)] prion of the yeast Saccharomyces cerevisiae was first identified by Brian Cox some 40 years ago as a non-Mendelian genetic element that modulated the efficiency of nonsense suppression. Following the suggestion by Reed Wickner in 1994 that such elements might be accounted for by invoking a prion-based model, it was subsequently established that the [PSI(+)] determinant was the prion form of the Sup35p protein. In this article, we review how a combination of classical genetic approaches and modern molecular and biochemical methods has provided conclusive evidence of the prion basis of the [PSI(+)] determinant. In so doing we have tried to provide a historical context, but also describe the results of more recent experiments aimed at elucidating the mechanism by which the [PSI(+)] (and other yeast prions) are efficiently propagated in dividing cells. While understanding of the [PSI(+)] prion and its mode of propagation has, and will continue to have, an impact on mammalian prion biology nevertheless the very existence of a protein-based mechanism that can have a beneficial impact on a cell's fitness provides equally sound justification to fully explore yeast prions.

Item Type: Article
Additional information: 1046-2023 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Amino Acid Sequence Cell Division/genetics/physiology Models, Biological Molecular Chaperones/metabolism Molecular Sequence Data Mutation/genetics Phenotype Prions/chemistry/*genetics/metabolism Saccharomyces cerevisiae/*genetics/growth & development/metabolism Saccharomyces cerevisiae Proteins/chemistry/*genetics/metabolism
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Mick Tuite
Date Deposited: 02 Sep 2008 23:06
Last Modified: 10 Jun 2014 11:11
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