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Protein 4.1G Regulates Cell Adhesion, Spreading and Migration of Mouse Embryo Fibroblasts through ?1 Integrin Pathway

Chen, Lixiang, Wang, Ting, Wang, Yaomei, Zhang, Jingxin, Qi, Yuanming, Weng, Haibo, Kang, Qiaozhen, Guo, Xinhua, Baines, Anthony J., Mohandas, Narla, and others. (2016) Protein 4.1G Regulates Cell Adhesion, Spreading and Migration of Mouse Embryo Fibroblasts through ?1 Integrin Pathway. Journal of Biological Chemistry, 291 . pp. 2170-2180. ISSN 0021-9258. E-ISSN 1083-351X. (doi:10.1074/jbc.M115.658591) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:54276)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://doi.org/10.1074/jbc.M115.658591

Abstract

Protein 4.1G is a membrane skeletal protein that can serve as an adapter between transmembrane proteins and the underlying membrane skeleton. The function of 4.1G remains largely unexplored. Here, using 4.1G knockout mouse embryonic fibroblasts (MEFs) as a model system, we explored the function of 4.1G in motile cells. We show that the adhesion, spreading, and migration of 4.1G?/? MEF cells are impaired significantly. We further show that, although the total cellular expression of ?1 integrin is unchanged, the surface expression of ?1 integrin and its active form are decreased significantly in 4.1G?/? MEF cells. Moreover, the phosphorylation of focal adhesion kinase, a downstream component of the integrin-mediated signal transduction pathway, is suppressed in 4.1G?/? MEF cells. Co-immunoprecipitation experiments and in vitro binding assays showed that 4.1G binds directly to ?1 integrin via its membrane-binding domain. These findings identified a novel role of 4.1G in cell adhesion, spreading, and migration in MEF cells by modulating the surface expression of ?1 integrin and subsequent downstream signal transduction.

Item Type: Article
DOI/Identification number: 10.1074/jbc.M115.658591
Uncontrolled keywords: adhesion; cytoskeleton; membrane; migration; phosphorylation; ?1 integrin; focal adhesion kinase; mouse embryonic, fibroblast; protein 4.1G; spreading
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 24 Feb 2016 16:41 UTC
Last Modified: 29 May 2019 17:01 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/54276 (The current URI for this page, for reference purposes)
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