Skip to main content

Classification of thermophilic actinobacteria isolated from arid desert soils, including the description of Amycolatopsis deserti sp. nov.

Busarakam, Kanungnid, Brown, Ros, Bull, Alan T., Tan, Geok Yuan Annie, Zucchi, Tiago D., da Silva, Leonardo José, de Souza, Wallace Rafael, Goodfellow, Michael (2016) Classification of thermophilic actinobacteria isolated from arid desert soils, including the description of Amycolatopsis deserti sp. nov. Antonie van Leeuwenhoek, 109 (2). pp. 319-334. ISSN 0003-6072. E-ISSN 1572-9699. (doi:10.1007/s10482-015-0635-8) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://doi.org/10.1007/s10482-015-0635-8

Abstract

The taxonomic position of 26 filamentous actinobacteria isolated from a hyper-arid Atacama Desert soil and 2 from an arid Australian composite soil was established using a polyphasic approach. All of the isolates gave the diagnostic amplification product using 16S rRNA oligonucleotide primers specific for the genus Amycolatopsis. Representative isolates had chemotaxonomic and morphological properties typical of members of the genus Amycolatopsis. 16S rRNA gene analyses showed that all of the isolates belong to the Amycolatopsis methanolica 16S rRNA gene clade. The Atacama Desert isolates were assigned to one or other of two recognised species, namely Amycolatopsis ruanii and Amycolatopsis thermalba, based on 16S rRNA gene sequence, DNA:DNA relatedness and phenotypic data; emended descriptions are given for these species. In contrast, the two strains from the arid Australian composite soil, isolates GY024T and GY142, formed a distinct branch at the periphery of the A. methanolica 16S rRNA phyletic line, a taxon that was supported by all of the tree-making algorithms and by a 100 % bootstrap value. These strains shared a high degree of DNA:DNA relatedness and have many phenotypic properties in common, some of which distinguished them from all of the constituent species classified in the A. methanolica 16S rRNA clade. Isolates GY024T and GY142 merit recognition as a new species within the A. methanolica group of thermophilic strains. The name proposed for the new species is Amycolatopsis deserti sp. nov.; the type strain is GY024T (=NCIMB 14972T = NRRL B-65266T).

Item Type: Article
DOI/Identification number: 10.1007/s10482-015-0635-8
Uncontrolled keywords: Amycolatopsis – Polyphasic taxonomy – Desert soils
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 24 Feb 2016 16:19 UTC
Last Modified: 01 Aug 2019 10:40 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/54275 (The current URI for this page, for reference purposes)
Bull, Alan T.: https://orcid.org/0000-0001-6223-7722
  • Depositors only (login required):