Activation of cross-reactive mucosal T and B cell responses in human nasopharynx associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines

Mullin, Jennifer and Ahmed, Muhammad S and Sharma, Ravi and Upile, Navdeep and Beer, Helen and Achar, Priya and Puksuriwong, Suttida and Ferrara, Francesca and Temperton, Nigel J. and McNamara, Paul S and Lambe, Teresa and Gilbert, Sarah C and Zhang, Qibo (2016) Activation of cross-reactive mucosal T and B cell responses in human nasopharynx associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines. Vaccine, . ISSN 0264-410X. (doi:https://doi.org/10.1016/j.vaccine.2016.02.028) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1016/j.vaccine.2016.02.028

Abstract

Recent efforts have been focused on the development of vaccines that could induce broad immunity against influenza virus, either through T cell responses to conserved internal antigens or B cell response to cross-reactive haemagglutinin (HA). We studied the capacity of Modified Vaccinia Ankara (MVA)-vectored influenza vaccines to induce cross-reactive immunity to influenza virus in human nasopharynx-associated lymphoid tissue (NALT) in vitro. Adenotonsillar cells were isolated and stimulated with MVA vaccines expressing either conserved nucleoprotein (NP) and matrix protein 1 (M1) (MVA-NP-M1) or pandemic H1N1 HA (MVA-pdmH1HA). The MVA vaccine uptake and expression, and T and B cell responses were analyzed. MVA-vectored vaccines were highly efficient infecting NALT and vaccine antigens were highly expressed by B cells. MVA-NP-M1 elicited T cell response with greater numbers of IFN?-producing CD4+ T cells and tissue-resident memory T cells than controls. MVA-pdmH1HA induced cross-reactive anti-HA antibodies to a number of influenza subtypes, in an age-dependent manner. The cross-reactive antibodies include anti-avian H5N1 and mainly target HA2 domain. CONCLUSION: MVA vaccines are efficient in infecting NALT and the vaccine antigen is highly expressed by B cells. MVA vaccines expressing conserved influenza antigens induce cross-reactive T and B cell responses in human NALT in vitro, suggesting the potential as mucosal vaccines for broader immunity against influenza.

Item Type: Article
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 18 Feb 2016 14:32 UTC
Last Modified: 02 Mar 2016 16:34 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/54236 (The current URI for this page, for reference purposes)
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