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Activation of cross-reactive mucosal T and B cell responses in human nasopharynx associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines

Mullin, Jennifer, Ahmed, Muhammad S, Sharma, Ravi, Upile, Navdeep, Beer, Helen, Achar, Priya, Puksuriwong, Suttida, Ferrara, Francesca, Temperton, Nigel J., McNamara, Paul S, and others. (2016) Activation of cross-reactive mucosal T and B cell responses in human nasopharynx associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines. Vaccine, . ISSN 0264-410X. (doi:10.1016/j.vaccine.2016.02.028) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/j.vaccine.2016.02.028

Abstract

Recent efforts have been focused on the development of vaccines that could induce broad immunity against influenza virus, either through T cell responses to conserved internal antigens or B cell response to cross-reactive haemagglutinin (HA). We studied the capacity of Modified Vaccinia Ankara (MVA)-vectored influenza vaccines to induce cross-reactive immunity to influenza virus in human nasopharynx-associated lymphoid tissue (NALT) in vitro. Adenotonsillar cells were isolated and stimulated with MVA vaccines expressing either conserved nucleoprotein (NP) and matrix protein 1 (M1) (MVA-NP-M1) or pandemic H1N1 HA (MVA-pdmH1HA). The MVA vaccine uptake and expression, and T and B cell responses were analyzed. MVA-vectored vaccines were highly efficient infecting NALT and vaccine antigens were highly expressed by B cells. MVA-NP-M1 elicited T cell response with greater numbers of IFN?-producing CD4+ T cells and tissue-resident memory T cells than controls. MVA-pdmH1HA induced cross-reactive anti-HA antibodies to a number of influenza subtypes, in an age-dependent manner. The cross-reactive antibodies include anti-avian H5N1 and mainly target HA2 domain. CONCLUSION: MVA vaccines are efficient in infecting NALT and the vaccine antigen is highly expressed by B cells. MVA vaccines expressing conserved influenza antigens induce cross-reactive T and B cell responses in human NALT in vitro, suggesting the potential as mucosal vaccines for broader immunity against influenza.

Item Type: Article
DOI/Identification number: 10.1016/j.vaccine.2016.02.028
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 18 Feb 2016 14:32 UTC
Last Modified: 29 May 2019 17:01 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/54236 (The current URI for this page, for reference purposes)
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