Induction of broad immunity by thermostabilised vaccines incorporated in dissolvable microneedles using novel fabrication methods

Vrdoljak, Anto, Allen, Evin A., Ferrara, Francesca, Temperton, Nigel J., Crean, Abina M., Moore, Anne C. (2016) Induction of broad immunity by thermostabilised vaccines incorporated in dissolvable microneedles using novel fabrication methods. Journal of controlled release, 225 . pp. 192-204. ISSN 0168-3659. E-ISSN 1873-4995. (doi:10.1016/j.jconrel.2016.01.019)

PDF - Author's Accepted Manuscript

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Download (884kB) Preview
[img]
Preview
PDF - Author's Accepted Manuscript
Restricted to Repository staff only
Contact us about this Publication Download (825kB)
[img]
Official URL
http://dx.doi.org/10.1016/j.jconrel.2016.01.019

Abstract

Dissolvable microneedle (DMN) patches for immunization have multiple benefits, including vaccine stability and ease-of-use. However, conventional DMN fabrication methods have several drawbacks. Here we describe a novel, microfluidic, drop dispensing-based dissolvable microneedle production method that overcomes these issues. Uniquely, heterogeneous arrays, consisting of microneedles of diverse composition, can be easily produced on the same patch. Robustness of the process was demonstrated by incorporating and stabilizing adenovirus and MVA vaccines. Clinically-available trivalent inactivated influenza vaccine (TIV) in DMN patches is fully stable for greater than 6months at 40°C. Immunization using low dose TIV-loaded DMN patches induced significantly higher antibody responses compared to intramuscular-based immunization in mice. TIV-loaded patches also induced a broader, heterosubtypic neutralizing antibody response. By addressing issues that will be faced in large-scale fill-finish DMN fabrication processes and demonstrating superior thermostable characteristics and immunogenicity, this study progresses the translation of this microneedle platform to eventual clinical deployment.

Item Type: Article
DOI/Identification number: 10.1016/j.jconrel.2016.01.019
Uncontrolled keywords: Microneedle; Influenza vaccine; Broadly-neutralizing antibody; Stability; Virus vector vaccine;
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 18 Jan 2016 21:58 UTC
Last Modified: 29 May 2019 16:52 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/53725 (The current URI for this page, for reference purposes)
  • Depositors only (login required):

Downloads

Downloads per month over past year