Skip to main content

Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR

Wagstaff, Jane L., Sadovskaya, Irina, Vinogradov, Evgeny, Jabbouri, Said, Howard, Mark J. (2008) Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR. Molecular Biosystems, 4 (2). pp. 170-174. ISSN 1742-206X. (doi:10.1039/b715242f) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:5355)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1039/b715242f

Abstract

We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired 1 H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6)-N-acetylglucosamine (PNAG) and poly(glycerol phosphate) teichoic acid (TA) carbohydrate polymer components in crude biofilm extracts from Staphylococcus epidermidis without the need for further sample purification and component separation. Biofilms are implicated in the barriers for resistance of microbes toward antibiotics and immune responses, therefore efficient rapid detection and quantification of key components are important to assist in the design of a clinical infection response.

Item Type: Article
DOI/Identification number: 10.1039/b715242f
Uncontrolled keywords: coagulase-negative staphylococci structural elucidation intercellular adhesin epidermidis rp62a strain polysaccharide metabonomics components aureus
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: M.J. Howard
Date Deposited: 13 Mar 2009 11:44 UTC
Last Modified: 28 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/5355 (The current URI for this page, for reference purposes)
  • Depositors only (login required):