Mattos dos Santos, Paula, Hall, Andrew J., Manesiotis, Panagiotis (2015) Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur. Journal of Chromatography B: Biomedical Sciences and Applications, . ISSN 1387-2273. (doi:10.1016/j.jchromb.2015.12.015) (KAR id:53519)
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Official URL: http://dx.doi.org/10.1016/j.jchromb.2015.12.015 |
Abstract
Molecularly Imprinted Polymers (MIPs) targeting tegafur, an anti-cancer 5-fluorouracil pro-drug, have been prepared by stoichiometric imprinting using 2,6-bis(acrylamido)pyridine (BAAPy) as the functional monomer. Solution association between tegafur and BAAPy was studied by 1H NMR titration, which confirmed the formation of 1:1 complexes with an affinity constant of 574±15 M?1 in CDCl3. Evaluation of the synthesised materials by HPLC and equilibrium rebinding experiments revealed high selectivity of the imprinted polymer for the pro-drug versus 5-fluorouracil and other competing analytes, with maximum imprinting factors of 25.3 and a binding capacity of 45.1 ?mol g?1. The synthesised imprinted polymer was employed in solid-phase extraction of the pro-drug using an optimised protocol that included a simple wash with the porogen used in the preparation of the material. Tegafur recoveries of up to 96% were achieved from aqueous samples and 92% from urine samples spiked with the template and three competing analytes. The results demonstrate the potential of the prepared polymers in the pre-concentration of tegafur from biological samples, which could be an invaluable tool in the monitoring of patient compliance and drug uptake and excretion.
Item Type: | Article |
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DOI/Identification number: | 10.1016/j.jchromb.2015.12.015 |
Uncontrolled keywords: | Molecularly Imprinted Polymers, Stoichiometric imprinting, Tegafur |
Subjects: | Q Science > QD Chemistry > Analytical Chemistry |
Divisions: | Divisions > Division of Natural Sciences > Medway School of Pharmacy |
Funders: | Organisations -1 not found. |
Depositing User: | Andrew Hall |
Date Deposited: | 17 Dec 2015 12:25 UTC |
Last Modified: | 04 Jul 2023 12:43 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/53519 (The current URI for this page, for reference purposes) |
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