Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur

Mattos dos Santos, Paula, Hall, Andrew J., Manesiotis, Panagiotis (2015) Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur. Journal of Chromatography B: Biomedical Sciences and Applications, . ISSN 1387-2273. (doi:10.1016/j.jchromb.2015.12.015)

PDF (Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur) - Author's Accepted Manuscript

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Download (743kB) Preview
[img]
Preview
PDF (Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur) - Author's Accepted Manuscript
Restricted to Repository staff only
Contact us about this Publication Download (1MB)
[img]
Official URL
http://dx.doi.org/10.1016/j.jchromb.2015.12.015

Abstract

Molecularly Imprinted Polymers (MIPs) targeting tegafur, an anti-cancer 5-fluorouracil pro-drug, have been prepared by stoichiometric imprinting using 2,6-bis(acrylamido)pyridine (BAAPy) as the functional monomer. Solution association between tegafur and BAAPy was studied by 1H NMR titration, which confirmed the formation of 1:1 complexes with an affinity constant of 574±15 M?1 in CDCl3. Evaluation of the synthesised materials by HPLC and equilibrium rebinding experiments revealed high selectivity of the imprinted polymer for the pro-drug versus 5-fluorouracil and other competing analytes, with maximum imprinting factors of 25.3 and a binding capacity of 45.1 ?mol g?1. The synthesised imprinted polymer was employed in solid-phase extraction of the pro-drug using an optimised protocol that included a simple wash with the porogen used in the preparation of the material. Tegafur recoveries of up to 96% were achieved from aqueous samples and 92% from urine samples spiked with the template and three competing analytes. The results demonstrate the potential of the prepared polymers in the pre-concentration of tegafur from biological samples, which could be an invaluable tool in the monitoring of patient compliance and drug uptake and excretion.

Item Type: Article
DOI/Identification number: 10.1016/j.jchromb.2015.12.015
Uncontrolled keywords: Molecularly Imprinted Polymers, Stoichiometric imprinting, Tegafur
Subjects: Q Science > QD Chemistry > Analytical Chemistry
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Andrew Hall
Date Deposited: 17 Dec 2015 12:25 UTC
Last Modified: 29 May 2019 16:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/53519 (The current URI for this page, for reference purposes)
Hall, Andrew J.: https://orcid.org/0000-0001-8849-7063
  • Depositors only (login required):

Downloads

Downloads per month over past year