Atherton, Paul, Stuchbury, Ben, Wang, De-Yao, Jethwa, Devina, Tsang, Ricky, Meiler-Rodriguez, Eugenia, Wang, Pengbo, Bate, Neil, Zent, Roy, Barsukov, Igor L, and others. (2015) Vinculin controls talin engagement with the actomyosin machinery. Nature Communications, 6 . pp. 1-12. ISSN 2041-1723. (doi:10.1038/ncomms10038) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication) | |
Official URL http://dx.doi.org/10.1038/ncomms10038 |
Abstract
The link between extracellular-matrix-bound integrins and intracellular F-actin is essential for cell spreading and migration. Here, we demonstrate how the actin-binding proteins talin and vinculin cooperate to provide this link. By expressing structure-based talin mutants in talin null cells, we show that while the C-terminal actin-binding site (ABS3) in talin is required for adhesion complex assembly, the central ABS2 is essential for focal adhesion (FA) maturation. Thus, although ABS2 mutants support cell spreading, the cells lack FAs, fail to polarize and exert reduced force on the surrounding matrix. ABS2 is inhibited by the preceding mechanosensitive vinculin-binding R3 domain, and deletion of R2R3 or expression of constitutively active vinculin generates stable force-independent FAs, although cell polarity is compromised. Our data suggest a model whereby force acting on integrin-talin complexes via ABS3 promotes R3 unfolding and vinculin binding, activating ABS2 and locking talin into an actin-binding configuration that stabilizes FAs.
Item Type: | Article |
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DOI/Identification number: | 10.1038/ncomms10038 |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculties > Sciences > School of Biosciences |
Depositing User: | Ben Goult |
Date Deposited: | 04 Dec 2015 14:21 UTC |
Last Modified: | 29 May 2019 16:36 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/52694 (The current URI for this page, for reference purposes) |
Goult, Benjamin T: | ![]() |
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