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A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration

Villari, G, Jayo, A, Zanet, J, Fitch, B, Serrels, B, Frame, M, Stramer, BM, Goult, Benjamin T, Parsons, M (2015) A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration. Journal of Cell Science, . pp. 1-32. (doi:10.1242/jcs.175760)

Abstract

Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown. Here we show for the first time that fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascin-dependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK) and Src, and that this signalling pathway lies downstream of fascin-microtubule association in the control of adhesion stability. These findings shed light on new non actin-dependent roles for fascin and may have implications for the design of therapies to target fascin in metastatic disease.

Item Type: Article
DOI/Identification number: 10.1242/jcs.175760
Subjects: Q Science > QH Natural history > QH581.2 Cell Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 28 Nov 2015 22:55 UTC
Last Modified: 29 May 2019 16:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/52502 (The current URI for this page, for reference purposes)
Goult, Benjamin T: https://orcid.org/0000-0002-3438-2807
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