Fabrication of scalable and structured tissue engineering scaffolds using water dissolvable sacrificial 3D printed moulds

Mohanty, Soumyaranjan and Larsen, Layla Bashir and Trifol, Jon and Szabo, Peter and Burri, Harsha Vardhan Reddy and Canali, Chiara and Dufva, Marin and Emnéus, Jenny and Wolff, Anders (2015) Fabrication of scalable and structured tissue engineering scaffolds using water dissolvable sacrificial 3D printed moulds. Materials Science and Engineering: C, 55 . pp. 569-578. ISSN 0928-4931. (doi:https://doi.org/10.1016/j.msec.2015.06.002) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1016/j.msec.2015.06.002

Abstract

One of the major challenges in producing large scale engineered tissue is the lack of ability to create large highly perfused scaffolds in which cells can grow at a high cell density and viability. Here, we explore 3D printed polyvinyl alcohol (PVA) as a sacrificial mould in a polymer casting process. The PVA mould network defines the channels and is dissolved after curing the polymer casted around it. The printing parameters determined the PVA filament density in the sacrificial structure and this density resulted in different stiffness of the corresponding elastomer replica. It was possible to achieve 80% porosity corresponding to about 150 cm2/cm3 surface to volume ratio. The process is easily scalable as demonstrated by fabricating a 75 cm3 scaffold with about 16,000 interconnected channels (about 1 m2 surface area) and with a channel to channel distance of only 78 ?m. To our knowledge this is the largest scaffold ever to be produced with such small feature sizes and with so many structured channels. The fabricated scaffolds were applied for in-vitro culturing of hepatocytes over a 12-day culture period. Smaller scaffolds (6 × 4 mm) were tested for cell culturing and could support homogeneous cell growth throughout the scaffold. Presumably, the diffusion of oxygen and nutrient throughout the channel network is rapid enough to support cell growth. In conclusion, the described process is scalable, compatible with cell culture, rapid, and inexpensive.

Item Type: Article
Subjects: T Technology
Divisions: Faculties > Sciences > School of Engineering and Digital Arts
Faculties > Sciences > School of Engineering and Digital Arts > Instrumentation, Control and Embedded Systems
Depositing User: Tina Thompson
Date Deposited: 26 Nov 2015 12:16 UTC
Last Modified: 27 Nov 2015 11:50 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/52452 (The current URI for this page, for reference purposes)
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