Hale, J. P., Marcelli, Gianluca, Parker, K. H., Winlove, C. Peter, Petrov, Peter G. (2007) Advanced red blood cell thermal fluctuation analysis: a new method to quantify membrane elasticity. European Biophysics Journal, 36 (S1). pp. 51-248. ISSN 0175-7571. (doi:10.1007/s00249-007-0178-7) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:51793)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1007/s00249-007-0178-7 |
Abstract
We propose a new experimental technique for red blood cell thermal
fluctuation analysis, which makes it possible to quantify the
cell membrane elastic moduli. The method is based on the comparison
of the mean-square fluctuations in the shape of cell equatorial
contours as observed using phase contrast microscopy to the equivalent
fluctuation spectrum acquired in a coarse grained molecular
dynamics simulation. The simulation is based on a network of virtual
particles which interact via a harmonic potential and a dihedral
angle potential and are subject to a constant volume and area constraints,
which gives rise to finite values for the membrane bending
and shear elastic moduli. Using this method, the elastic properties
of individual red blood cells can be measured and their changes can
be easily monitored in response to changing environmental conditions
(temperature, solute concentration, osmotic pressure changes).
We also present evidence of increased bending rigidity in red blood
cells from diabetic individuals.
Item Type: | Article |
---|---|
DOI/Identification number: | 10.1007/s00249-007-0178-7 |
Subjects: | Q Science > QC Physics > QC173.45 Condensed Matter |
Divisions: | Divisions > Division of Computing, Engineering and Mathematical Sciences > School of Engineering and Digital Arts |
Depositing User: | Gianluca Marcelli |
Date Deposited: | 12 Nov 2015 17:24 UTC |
Last Modified: | 16 Nov 2021 10:21 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/51793 (The current URI for this page, for reference purposes) |
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