King, Barnabas, Temperton, Nigel J., Grehan, Keith, Scott, Simon D., Wright, Edward, Tarr, Alexander, Daly, Janet (2015) Technical considerations for the generation of novel pseudotyped viruses. Future Virology, 11 (1). pp. 47-59. ISSN 1746-0794. E-ISSN 1746-0808. (doi:10.2217/fvl.15.106) (KAR id:51318)
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| Official URL http://dx.doi.org/10.2217/fvl.15.106 |
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Abstract
A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein of the virus as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate PVs, with a focus on approaches to improve the efficacy of PV generation.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.2217/fvl.15.106 |
| Uncontrolled keywords: | envelope glycoproteins; pseudoparticles; pseudotyped virus; pseudotypes |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Divisions: | Divisions > Division of Natural Sciences > Medway School of Pharmacy |
| Depositing User: | Nigel Temperton |
| Date Deposited: | 01 Nov 2015 10:10 UTC |
| Last Modified: | 16 Feb 2021 13:29 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/51318 (The current URI for this page, for reference purposes) |
| Temperton, Nigel J.: |  https://orcid.org/0000-0002-7978-3815 |
| Scott, Simon D.: | https://orcid.org/0000-0002-8290-0461 |
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