Technical considerations for the generation of novel pseudotyped viruses

King, Barnabas and Temperton, Nigel J. and Grehan, Keith and Scott, Simon D. and Wright, Edward and Tarr, Alexander and Daly, Janet (2015) Technical considerations for the generation of novel pseudotyped viruses. Future Virology, 11 (1). pp. 47-59. ISSN 1746-0794. E-ISSN 1746-0808. (doi:https://doi.org/10.2217/fvl.15.106) (Full text available)

Abstract

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein of the virus as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate PVs, with a focus on approaches to improve the efficacy of PV generation.

Item Type:	Article
Uncontrolled keywords:	envelope glycoproteins; pseudoparticles; pseudotyped virus; pseudotypes
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculties > Sciences > Medway School of Pharmacy
Depositing User:	Nigel Temperton
Date Deposited:	01 Nov 2015 10:10 UTC
Last Modified:	04 Dec 2017 10:53 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/51318 (The current URI for this page, for reference purposes)
Temperton, Nigel J.:	https://orcid.org/0000-0002-7978-3815
Scott, Simon D.:	https://orcid.org/0000-0002-8290-0461