King, Barnabas, Temperton, Nigel J., Grehan, Keith, Scott, Simon D., Wright, Edward, Tarr, Alexander, Daly, Janet (2015) Technical considerations for the generation of novel pseudotyped viruses. Future Virology, 11 (1). pp. 47-59. ISSN 1746-0794. E-ISSN 1746-0808. (doi:10.2217/fvl.15.106) (KAR id:51318)
|
PDF
Pre-print
Language: English |
||
|
Download (619kB)
Preview
|
|
|
|
This file may not be suitable for user of assistive technology. Request an accessible format. |
||
| Official URL http://dx.doi.org/10.2217/fvl.15.106 |
||
Abstract
A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein of the virus as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate PVs, with a focus on approaches to improve the efficacy of PV generation.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.2217/fvl.15.106 |
| Uncontrolled keywords: | envelope glycoproteins; pseudoparticles; pseudotyped virus; pseudotypes |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Divisions: | Faculties > Sciences > Medway School of Pharmacy |
| Depositing User: | Nigel Temperton |
| Date Deposited: | 01 Nov 2015 10:10 UTC |
| Last Modified: | 29 May 2019 16:14 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/51318 (The current URI for this page, for reference purposes) |
| Temperton, Nigel J.: |  https://orcid.org/0000-0002-7978-3815 |
| Scott, Simon D.: | https://orcid.org/0000-0002-8290-0461 |
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):
Altmetric
Altmetric