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Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects.

Bill, Roslyn M, von der Haar, Tobias (2015) Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects. Current Opinion in Structural Biology, 32 . pp. 147-155. ISSN 0959-440X. (doi:10.1016/j.sbi.2015.04.003) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://doi.org/10.1016/j.sbi.2015.04.003

Abstract

Membrane protein structural biology is critically dependent upon the supply of high-quality protein. Over the last few years, the value of crystallising biochemically characterised, recombinant targets that incorporate stabilising mutations has been established. Nonetheless, obtaining sufficient yields of many recombinant membrane proteins is still a major challenge. Solutions are now emerging based on an improved understanding of recombinant host cells; as a ‘cell factory’ each cell is tasked with managing limited resources to simultaneously balance its own growth demands with those imposed by an expression plasmid. This review examines emerging insights into the role of translation and protein folding in defining high-yielding recombinant membrane protein production in a range of host cells.

Item Type: Article
DOI/Identification number: 10.1016/j.sbi.2015.04.003
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 22 Oct 2015 10:32 UTC
Last Modified: 01 Aug 2019 10:39 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/51193 (The current URI for this page, for reference purposes)
von der Haar, Tobias: https://orcid.org/0000-0002-6031-9254
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