Skip to main content

Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1.

Gewiss Mogensen, Estelle, Janbon, Guihem, Chaloupka, James, Steegborn, Clemens, Fu, Man Shun, Moyrand, Frédérique, Klengel, Torsten, Pearson, David S., Geeves, Michael A., Buck, Jochen, and others. (2006) Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1. Eukaryotic Cell, 5 (1). pp. 103-111. ISSN 1535-9778. E-ISSN 1535-9786. (doi:10.1128/EC.5.1.103-111.2006) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:51)

Language: English

Restricted to Repository staff only
[thumbnail of Gewiss_Mogensen_et_al_2006,_Eukaryot_Cell,_5,_103-111.pdf]
Official URL


Cryptococcus neoformans, a fungal pathogen of humans, causes fatal meningitis in immunocompromised patients. Its virulence is mainly determined by the elaboration of a polysaccharide capsule surrounding its cell wall. During its life, C. neoformans is confronted with and responds to dramatic variations in CO2 concentrations; one important morphological change triggered by the shift from its natural habitat (0.033% CO2) to infected hosts (5% CO2) is the induction of capsule biosynthesis. In cells, CO2 is hydrated to bicarbonate in a spontaneous reaction that is accelerated by carbonic anhydrases. Here we show that C. neoformans contains two beta-class carbonic anhydrases, Can1 and Can2. We further demonstrate that CAN2, but not CAN1, is abundantly expressed and essential for the growth of C. neoformans in its natural environment, where CO2 concentrations are limiting. Structural studies reveal that Can2 forms a homodimer in solution. Our data reveal Can2 to be the main carbonic anhydrase and suggest a physiological role for bicarbonate during C. neoformans growth. Bicarbonate directly activates the C. neoformans Cac1 adenylyl cyclase required for capsule synthesis. We show that this specific activation is optimal at physiological pH.

Item Type: Article
DOI/Identification number: 10.1128/EC.5.1.103-111.2006
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 19 Dec 2007 17:50 UTC
Last Modified: 16 Nov 2021 09:38 UTC
Resource URI: (The current URI for this page, for reference purposes)
Geeves, Michael A.:
  • Depositors only (login required):