Skip to main content

Combining confocal microscopy and optical coherence tomography for imaging in developmental biology

Bradu, A. and Ma, L. and Bloor, J. and Podoleanu, A. (2008) Combining confocal microscopy and optical coherence tomography for imaging in developmental biology. In: Biophotonics: Photonic Solutions for Better Health Care. Proceedings of SPIE . SPIE (The International Society for Optical Engineering). ISBN 978-0-8194-7189-5. (doi:10.1117/12.781041) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1117/12.781041

Abstract

In-vivo Optical Coherence Tomography (OCT) imaging of the fruit fly Drosophila melanogaster larval heart allows non invasive visualizations and assesment of its cardiac functions. To image Drosophila melanogaster heart, we have developed a dedicated imaging instrument able to provide simultaneous Optical Coherence Tomography (OCT) and Laser Confocal Scanning Microscopy (LCSM) or Laser Scanning Fluorescence Microscopy (LSFM) images and can be used to produce B-scan OCT images. With this dual imaging system, the image of heart can be easily located in the specimen and the change of the heart shape in a cardiac cycle monitored. This technique therefore provides an excellent tool for large scale screen of candidate genes responsible for the contractility of the Drosophila heart. As this technique can also image the dynamic process of the heartbeat in a non-invasive fashion, it provides a new avenue to study the physiology of the heart function. En-face and B-scan OCT images of the Drosophila melanogaster heart showing its chambers have been obtained with our imaging instruments. Our results are consistent with detailed anatomical studies from the literature.

Item Type: Book section
DOI/Identification number: 10.1117/12.781041
Uncontrolled keywords: Developmental biology; Drosophila melanogaster; Heart; Imaging; Larvae; Laser scanning microscopy; Optical coherence tomography
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical coherence tomography
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Giles Tarver
Date Deposited: 24 Jul 2015 13:31 UTC
Last Modified: 26 Jul 2019 11:28 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49725 (The current URI for this page, for reference purposes)
  • Depositors only (login required):