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Using en-face optical coherence tomography to analyse gene function in Drosophila Melanogaster larval heart

Bradu, Adrian, Ma, L., Bloor, J., Podoleanu, Adrian G.H. (2008) Using en-face optical coherence tomography to analyse gene function in Drosophila Melanogaster larval heart. In: SPIE Proceedings Series. Proceedings of SPIE - The International Society for Optical Engineering. 7139. Spie-Int Soc Optical Engineering ISBN 978-0-8194-7380-6. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
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Abstract

In-vivo Optical Coherence Tomography (OCT) imaging of the fruit fly Drosophila Melanogaster larval heart allows non invasive visualizations and assessment of its cardiac function. In order to image Drosophila heart, we have developed a dedicated imaging instrument able to provide simultaneous OCT and Laser Scanning Confocal Microscopy (LSCM) images. With this dual imaging system, the heart can easily be located and visualised within the specimen and the change of the heart shape in a cardiac cycle monitored. Here we have used targeted gene expression to knockdown the myospheroid (mys) gene in the larval heart using a specific RNAi construct. By knocking down a β integrin subunit encoded by mys we have recorded an enlarged heart chamber in both diastolic and systolic states. Also, the fraction of reduction of the chamber diameter was smaller in the knockdown heart. These phenotypic differences indicate that impaired cardiac contractility occurs in the heart where the integrin gene express level is reduced. At our knowledge, this is for the first time when it is shown that integrins have a direct relationship to a dilated heart defect.

Item Type: Conference or workshop item (Proceeding)
Uncontrolled keywords: Cardiomyopathy, Developmental biology, Drosophila melanogaster, Heart, Larva, Laser scanning microscopy, Optical coherence tomography, Cardiomyopathy, Developmental biology, Drosophila melanogaster, Heart, Larva, Laser scanning microscopy, Optical coherence tomography,
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical coherence tomography
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Giles Tarver
Date Deposited: 24 Jul 2015 11:34 UTC
Last Modified: 29 May 2019 14:54 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49712 (The current URI for this page, for reference purposes)
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