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Sequential en-face optical coherence tomography imaging and monitoring of Drosophila Melanogaster larval heart

Bradu, A. and Ma, Lisha and Bloor, J. and Podoleanu, A.Gh. (2009) Sequential en-face optical coherence tomography imaging and monitoring of Drosophila Melanogaster larval heart. In: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIII. Proceedings of SPIE . SPIE. ISBN 978-0-8194-7414-8. (doi:10.1117/12.808159) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1117/12.808159

Abstract

This article demonstrates two modalities to acquire information on cardiac function in larval Drosophila Melanogaster: in-vivo imaging and heartbeat monitoring. To achieve these goals a dedicated imaging instrument able to provide simultaneous en-face Optical Coherence Tomography (OCT) and Laser Scanning Confocal Microscopy (LSCM) images has been developed. With this dual imaging system, the heart can easily be located and visualised within the specimen and the change of the heart shape in a cardiac cycle monitored. The system can easily be switched to a stethoscopic regime, simply by interrupting the scanning of the light beam across the sample, after selecting the point of interest in the imaging regime. Here we have used targeted gene expression to knockdown the myospheroid (mys) gene in the larval heart using a specific RNAi construct. By knocking down a β integrin subunit encoded by mys we have recorded an enlarged heart chamber in both diastolic and systolic states. Also, the fraction of reduction of the chamber diameter was smaller in the knockdown heart. These phenotypic differences indicate that impaired cardiac contractility occurs in the heart where the integrin gene express level is reduced. As far as we are aware, this is for the first time when it is shown in Drosophila that integrins have a direct relationship to a dilated heart defect, and conseqThis article demonstrates two modalities to acquire information on cardiac function in larval Drosophila Melanogaster: in-vivo imaging and heartbeat monitoring. To achieve these goals a dedicated imaging instrument able to provide simultaneous en-face Optical Coherence Tomography (OCT) and Laser Scanning Confocal Microscopy (LSCM) images has been developed. With this dual imaging system, the heart can easily be located and visualised within the specimen and the change of the heart shape in a cardiac cycle monitored. The system can easily be switched to a stethoscopic regime, simply by interrupting the scanning of the light beam across the sample, after selecting the point of interest in the imaging regime. Here we have used targeted gene expression to knockdown the myospheroid (mys) gene in the larval heart using a specific RNAi construct. By knocking down a β integrin subunit encoded by mys we have recorded an enlarged heart chamber in both diastolic and systolic states. Also, the fraction of reduction of the chamber diameter was smaller in the knockdown heart. These phenotypic differences indicate that impaired cardiac contractility occurs in the heart where the integrin gene express level is reduced. As far as we are aware, this is for the first time when it is shown in Drosophila that integrins have a direct relationship to a dilated heart defect, and consequently we demonstrate the utility of Drosophila as model for the study of vertebrate heart disease. By monitoring the heartbeat we also demonstrated a reduction of the heart rate in Tropomyosin mutant compared to the wild type larva.uently we demonstrate the utility of Drosophila as model for the study of vertebrate heart disease. By monitoring the heartbeat we also demonstrated a reduction of the heart rate in Tropomyosin mutant compared to the wild type larva.

Item Type: Book section
DOI/Identification number: 10.1117/12.808159
Additional information: Unmapped bibliographic data: C7 - 71680G [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - Progr. Biomed. Opt. Imaging Proc. SPIE [Field not mapped to EPrints] AD - School of Physical Sciences, University of Kent, CT2 7NH, Canterbury, United Kingdom [Field not mapped to EPrints] AD - Dept. of Biosciences, University of Kent, CT2 7NJ, Canterbury, United Kingdom [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] C3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE [Field not mapped to EPrints]
Uncontrolled keywords: Optical Coherence Tomography, Drosophila Melanogaster, larval heart, integrin, RNAi, Cardiac contractilities, Cardiac cycles, Cardiac functions, Heart defects, Heart disease, Heart rates, Imaging instruments, In-vivo imaging, Integrins, Laser-scanning confocal microscopies, Light beams, Optical Coherence Tomography, Drosophila Melanogaster, larval heart, integrin, RNAi, Phenotypic differences, Point of interests, Tropomyosin, Wild types, Confocal microscopy, Gene expression, Optical microscopy, Optical tomography, Optoelectronic devices, Scanning, Tomography, Coherent light
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical coherence tomography
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Giles Tarver
Date Deposited: 14 Jul 2015 15:26 UTC
Last Modified: 29 Jul 2019 13:27 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49471 (The current URI for this page, for reference purposes)
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