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In vivo in situ en face optical coherence tomography imaging of chick embryos

Leitner, Michael and Castanheira, Joana and Ferreira, Luís and Ferreira, Mónica and Palmeirim, Isabel and Rosa, Carla C. and Podoleanu, Adrian Gh. (2009) In vivo in situ en face optical coherence tomography imaging of chick embryos. In: Optical Coherence Tomography and Coherence Techniques IV. Proceedings of SPIE . SPIE. ISBN 978-0-8194-7648-7. (doi:10.1117/12.831859) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1117/12.831859

Abstract

Chick embryos are among the most studied species in development biology because they are easily obtained, highly accessible and present a similar development to that of humans. Normally morphological studies are carried out with confocal microscopy, however in-situ imaging is impossible and in- vivo imaging can only be performed with great difficulty. For confocal microscopy the embryo has to be studied outside the egg, what generally also means a short life expectancy of the embryo. Additionally, extracting the embryo of the egg precludes the possibility of studying its development in its natural environment. In this paper it is shown that en-face optical coherence tomography (en-face OCT) is a possible solution to overcome these difficulties allowing for an in-situ and in-vivo study over a timescale of several days. With en-face OCT it is possible to accompany the development of one single embryo over several days and to acquire high resolution and axially resolved images.

Item Type: Book section
DOI/Identification number: 10.1117/12.831859
Additional information: Unmapped bibliographic data: C7 - 73720Y [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - Progr. Biomed. Opt. Imaging Proc. SPIE [Field not mapped to EPrints] AD - Faculty of Science, University of Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal [Field not mapped to EPrints] AD - INESC Porto, Instituto de Engenharia de Sistemas e Computadores-Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal [Field not mapped to EPrints] AD - Applied Optics Group, School of Physical Sciences, University of Kent at Canterbury, Canterbury, CT2 7NH, United Kingdom [Field not mapped to EPrints] AD - Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] A4 - Optical Society of America; The Society of Photo-Optical Instrumentation Engineers (SPIE); Photonics4Life-European Network of Excellence for Biophotonics; Air Force Office of Scientific Research [Field not mapped to EPrints] C3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE [Field not mapped to EPrints]
Uncontrolled keywords: Chick embryos, Development biology, En-face OCT, In-vivo imaging, Optical coherence tomography, Time-domain OCT, Chick embryo, Development biology, En-face OCT, In-Vivo imaging, Time domain, Confocal microscopy, Time domain analysis, Tomography
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical coherence tomography
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Giles Tarver
Date Deposited: 14 Jul 2015 15:35 UTC
Last Modified: 26 Jul 2019 11:23 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49454 (The current URI for this page, for reference purposes)
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