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Time domain optical coherence tomography investigation of bone matrix interface in rat femurs

Rusu, L.C., Negrutiu, M.-L., Sinescu, C., Hoinoiu, B., Topala, F.-I., Duma, V.-F., Rominu, M., Podoleanu, Adrian G.H. (2013) Time domain optical coherence tomography investigation of bone matrix interface in rat femurs. In: SPIE Proceedings Series. 5th International Symposium on Photoelectronic Detection and Imaging. 8914. Spie-Int Soc Optical Engineering ISBN 978-0-8194-9783-3. (doi:10.1117/12.2036345) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:49354)

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The materials used to fabricate scaffolds for tissue engineering are derived from synthetic polymers, mainly from the polyester family, or from natural materials (e.g., collagen and chitosan). The mechanical properties and the structural properties of these materials can be tailored by adjusting the molecular weight, the crystalline state, and the ratio of monomers in the copolymers. Quality control and adjustment of the scaffold manufacturing process are essential to achieve high standard scaffolds. Most scaffolds are made from highly crystalline polymers, which inevitably result in their opaque appearance. Their 3-D opaque structure prevents the observation of internal uneven surface structures of the scaffolds under normal optical instruments, such as the traditional light microscope. The inability to easily monitor the inner structure of scaffolds as well as the interface with the old bone poses a major challenge for tissue engineering: it impedes the precise control and adjustment of the parameters that affect the cell growth in response to various mimicked culture conditions. The aim of this paper is to investigate the interface between the femur rat bone and the new bone that is obtained using a method of tissue engineering that is based on different artificial matrixes inserted in previously artificially induced defects. For this study, 15 rats were used in conformity with ethical procedures. In all the femurs a round defect was induced by drilling with a 1 mm spherical Co-Cr surgical drill. The matrixes used were Bioss and 4bone. These materials were inserted into the induced defects. The femurs were investigated at 1 week, 1 month, 2 month and three month after the surgical procedures. The interfaces were examined using Time Domain (TD) Optical Coherence Tomography (OCT) combined with Confocal Microscopy (CM). The optical configuration uses two single mode directional couplers with a superluminiscent diode as the source centered at 1300 nm. The scanning procedure is similar to that used in any CM, where the fast scanning is en-face (line rate) and the scanning in depth is much slower (at the frame rate). The results showed open interfaces due to the insufficient healing process, as well as closed interfaces due to a new bone formation inside the defect. The conclusion of this study is that TD-OCT can act as a valuable tool in the investigation of the interface between the old bone and the one that has been newly induced due to the osteoinductive process. © 2013 SPIE.

Item Type: Conference or workshop item (Proceeding)
DOI/Identification number: 10.1117/12.2036345
Uncontrolled keywords: confocal microscopy, femurs, healing process, optical coherence tomography, rat bone, time domain, Tissue engineering, Bone, Confocal microscopy, Crystalline materials, Defects, Mechanical properties, Multilayers, Natural polymers, Polymers, Rats, Scaffolds (biology), Scanning, Tissue, Tissue engineering, Control and adjustment, femurs, Healing process, Scaffold manufacturing, Scaffolds for tissue engineering, Superluminiscent diode, Time domain, Time domain optical coherence tomography, Optical tomography
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical instruments
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: Giles Tarver
Date Deposited: 16 Jul 2015 09:48 UTC
Last Modified: 16 Feb 2021 13:26 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Podoleanu, Adrian G.H..

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