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Different matrix evaluation for the bone regeneration of rats' femours using time domain optical coherence tomography

Rusu, Laura-Cristina and Negrutiu, Meda Lavinia and Sinescu, Cosmin and Hoinoiu, Bogdan and Zaharia, Cristian and Ardelean, Lavinia and Duma, Virgil-Florin and Podoleanu, Adrian Gh. (2014) Different matrix evaluation for the bone regeneration of rats' femours using time domain optical coherence tomography. In: Fifth International Conference on Lasers in Medicine: Biotechnologies Integrated in Daily Medicine. Proceedings of SPIE . SPIE, Bellingham, Washington. ISBN 978-0-8194-9817-5. (doi:10.1117/12.2045849) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://www.dx.doi.org/10.1117/12.2045849

Abstract

The osteoconductive materials are important in bone regeneration procedures. Three dimensional (3D) reconstructions were obtained from the analysis. The aim of this study is to investigate the interface between the femur rat bone and the new bone that is obtained using a method of tissue engineering that is based on two artificial matrixes inserted in previously artificially induced defects. For this study, under strict supervision 20 rats were used in conformity with ethical procedures. In all the femurs a round defect was induced by drilling with a 1 mm spherical Co-Cr surgical drill. The matrixes used were IngeniOss (for ten samples) and 4Bone(for the other ten samples). These materials were inserted into the induced defects. The femurs were investigated at 1 month, after the surgical procedures. The interfaces were examined using Time Domain (TD) Optical Coherence Tomography (OCT) combined with Confocal Microscopy (CM). The scanning procedure is similar to that used in any CM, where the fast scanning is en-face (line rate) and the scanning in depth is much slower (at the frame rate). The optical configuration uses two single mode directional couplers with a superluminiscent diode as the source centered at 1300 nm. The results showed open interfaces due to the insufficient healing process, as well as closed interfaces due to a new bone formation inside the defect. The conclusion of this study is that TD-OCT can act as a valuable tool in the investigation of the interface between the old bone and the one that has been newly created due to the osteoinductive process. The TD-OCT has proven a valuable tool for the non-invasive evaluation of the matrix bone interfaces. © 2014 Copyright SPIE.

Item Type: Book section
DOI/Identification number: 10.1117/12.2045849
Additional information: Unmapped bibliographic data: C7 - 89250V [EPrints field already has value set] LA - English [Field not mapped to EPrints] J2 - Progr. Biomed. Opt. Imaging Proc. SPIE [Field not mapped to EPrints] AD - Faculty of Dentistry, University of Medicine and Pharmacy Victor Babes, Timisoara, Romania [Field not mapped to EPrints] AD - 3OM Optomechatronics Group, Aurel Vlaicu University of Arad, Romania [Field not mapped to EPrints] AD - Applied Optics Group, School of Physical Sciences, University of Kent, Canterbury, United Kingdom [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] A4 - Romanian Society for Lasers in Dentistry (SRLS) [Field not mapped to EPrints] C3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE [Field not mapped to EPrints]
Uncontrolled keywords: Bone regeneration, confocal microscopy, femurs, healing process, matrix, optical coherence tomography, time domain, Biotechnology, Bone, Confocal microscopy, Defects, Matrix algebra, Rats, Scanning, Three dimensional, Time domain analysis, Tissue engineering, Tools, Bone regeneration, femurs, Healing process, Osteoconductive materials, Superluminiscent diode, Three-dimensional (3-D) reconstruction, Time domain, Time domain optical coherence tomography, Optical tomography
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General) > R857.O6 Optical coherence tomography
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Giles Tarver
Date Deposited: 15 Jul 2015 09:05 UTC
Last Modified: 24 Jul 2019 15:28 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49314 (The current URI for this page, for reference purposes)
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