Oxygenase and Carboxylase Activities of RuBP Carboxylase from Wheat Leaves

Boyle, Frances A. and KEYS, A. J. (1983) Oxygenase and Carboxylase Activities of RuBP Carboxylase from Wheat Leaves. In: Advances in Photosynthesis Research, August 1-6, 1983, Brussels, Belgium. (doi:https://doi.org/10.1007/978-94-017-4973-2_174) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1007/978-94-017-4973-2_174

Abstract

The oxygenase reaction catalysed by RuBP carboxylase initiates a metabolic sequence responsible for photorespiration (Bowes et al. 1972). Studies with inhibitors and with mutants show that control of photorespiration can have beneficial effects only if it is achieved by decreasing the oxygenase activity of RuBP carboxylase (Servaites, Ogren, 1977; Somerville, Ogren, 1982). No corroborated evidence is available for carboxylation of RuBP without susceptibility to oxygenation. If oxygenase activity and photorespiration have a function in plants, then regulation by changing levels of metabolic intermediates might be expected. This was investigated in a test system containing purified RuBP carboxylase from wheat with RuBP and rate-limiting concentrations of HCO3 − and O2. The sensitivity of the ratio vc:vo to pH (CO2 concentration), the presence of carbonic anhydrase (CO2 availability) and the presence of Mn2+ is illustrated to validate the methods used.

Item Type: Conference or workshop item (Proceeding)
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Frances Mansfield
Date Deposited: 09 Jul 2015 08:14 UTC
Last Modified: 10 Jul 2015 08:47 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/49285 (The current URI for this page, for reference purposes)
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