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The Phyre2 web portal for protein modeling, prediction and analysis

Kelley, Lawrence A., Mezulis, Stefans, Yates, Christopher M., Wass, Mark N., Sternberg, Michael J. E. (2015) The Phyre2 web portal for protein modeling, prediction and analysis. Nature protocols, 10 (6). pp. 845-858. ISSN 1750-2799. (doi:10.1038/nprot.2015.053) (KAR id:49224)


Phyre2 is a suite of tools available on the web to predict and analyze protein structure, function and mutations. The focus of Phyre2 is to provide biologists with a simple and intuitive interface to state-of-the-art protein bioinformatics tools. Phyre2 replaces Phyre, the original version of the server for which we previously published a paper in Nature Protocols. In this updated protocol, we describe Phyre2, which uses advanced remote homology detection methods to build 3D models, predict ligand binding sites and analyze the effect of amino acid variants (e.g., nonsynonymous SNPs (nsSNPs)) for a user's protein sequence. Users are guided through results by a simple interface at a level of detail they determine. This protocol will guide users from submitting a protein sequence to interpreting the secondary and tertiary structure of their models, their domain composition and model quality. A range of additional available tools is described to find a protein structure in a genome, to submit large number of sequences at once and to automatically run weekly searches for proteins that are difficult to model. The server is available at A typical structure prediction will be returned between 30 min and 2 h after submission.

Item Type: Article
DOI/Identification number: 10.1038/nprot.2015.053
Subjects: Q Science > Q Science (General)
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mark Wass
Date Deposited: 06 Jul 2015 11:18 UTC
Last Modified: 10 Dec 2022 13:09 UTC
Resource URI: (The current URI for this page, for reference purposes)

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