Skip to main content
Kent Academic Repository

A GAS-like gene family in the pathogenic fungus Candida glabrata.

Weig, Michael, Haynes, Ken, Rogers, Thomas R., Kurzai, Oliver, Frosch, Matthias, Mühlschlegel, Fritz A. (2001) A GAS-like gene family in the pathogenic fungus Candida glabrata. Microbiology, 147 . pp. 2007-2019. ISSN 0002-4564. (doi:10.1099/00221287-147-8-2007) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:48)

PDF
Language: English

Restricted to Repository staff only
[thumbnail of Weig_et_al_2001,_Microbiology_147_2007-2019.pdf]
Official URL:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...

Abstract

In fungi, the cell wall plays a major role in host-pathogen interactions. Despite this, little is known about the molecular basis of cell wall assembly in Candida glabrata, which has emerged as the second most common cause of systemic candidosis. A C. glabrata gene family, CgGAS1-3, that shares significant homologies with both the GAS1 gene of Saccharomyces cerevisiae, which is necessary for cell wall assembly, and the pH-regulated genes PHR1 and PHR2 of Candida albicans, which are involved in cell wall assembly and required for virulence, has been cloned. Among the members of this family, CgGAS1-3 display a unique expression pattern. Both CgGAS1 and CgGAS2 are constitutively expressed. In contrast, CgGAS3 transcript was not detectable under any of the assayed conditions. The C. glabrata actin gene, CgACT1, has also been cloned to be used as a meaningful loading control in Northern blots. CgGAS1 and CgGAS2 were deleted by two different methodological approaches. A rapid PCR-based strategy by which gene disruption was achieved with short regions of homology (50 bp) was applied successfully to C. glabrata. DeltaCggas1 or DeltaCggas2 cells demonstrated similar aberrant morphologies, displaying an altered bud morphology and forming floccose aggregates. These phenotypes suggest a role for CgGAS1 and CgGAS2 in cell wall biosynthesis. Further evidence for this hypothesis was obtained by successful functional complementation of a gas1 null mutation in S. cerevisiae with the C. glabrata CgGAS1 or CgGAS2 gene.

Item Type: Article
DOI/Identification number: 10.1099/00221287-147-8-2007
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 19 Dec 2007 17:49 UTC
Last Modified: 05 Nov 2024 09:29 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/48 (The current URI for this page, for reference purposes)

University of Kent Author Information

Mühlschlegel, Fritz A..

Creator's ORCID:
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.