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Fluorescence to study the ATPase mechanism of motor proteins

Toseland, Christopher P. (2014) Fluorescence to study the ATPase mechanism of motor proteins. In: Toseland, Christopher P. and Fili, Natalia, eds. Fluorescent Methods for Molecular Motors. Experientia Supplementum, 105 . Springer, pp. 67-86. ISBN 978-3-0348-0855-2. E-ISBN 978-3-0348-0856-9. (doi:10.1007/978-3-0348-0856-9) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:47845)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/978-3-0348-0856-9

Abstract

This chapter provides an overview of different methodologies to dissect the ATPase mechanism of motor proteins. The use of ATP is fundamental to how these molecular engines work and how they can use the energy to perform various cellular roles. Rapid reaction and single-molecule techniques will be discussed to monitor reactions in real time through the application of fluorescence intensity, anisotropy and FRET. These approaches utilise fluorescent nucleotides and biosensors. While not every technique may be suitable for your motor protein, the different ways to determine the ATPase mechanism should allow a good evaluation of the kinetic parameters.

Item Type: Book section
DOI/Identification number: 10.1007/978-3-0348-0856-9
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Chris Toseland
Date Deposited: 07 Apr 2015 12:00 UTC
Last Modified: 29 May 2019 14:23 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/47845 (The current URI for this page, for reference purposes)
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