Pseudotype-based neutralization assays for influenza: a systematic analysis

Carnell, George W. and Ferrara, Francesca and Grehan, Keith and Thompson, Craig P. and Temperton, Nigel J. (2015) Pseudotype-based neutralization assays for influenza: a systematic analysis. Frontiers in Immunology, 6 . ISSN 1664-3224. E-ISSN 1664-3224. (doi:https://doi.org/10.3389/fimmu.2015.00161) (Full text available)

Abstract

The use of vaccination against the influenza virus remains the most effective method of mitigating the significant morbidity and mortality caused by this virus. Antibodies elicited by currently licensed influenza vaccines are predominantly hemagglutination-inhibition (HI)-competent antibodies that target the globular head of HA thus inhibiting influenza virus entry into target cells. These antibodies predominantly confer homosubtypic/strain specific protection and only rarely confer heterosubtypic protection. However, recent academia or pharma-led R&D towards the production of a "universal vaccine" has centered on the elicitation of antibodies directed against the stalk of the influenza HA that has been shown to confer broad protection across a range of different subtypes (H1 to H16). The accurate and sensitive measurement of antibody responses elicited by these "next-generation" influenza vaccines is however hampered by the lack of sensitivity of the traditional influenza serological assays hemagglutinin inhibition (HI), single radial hemolysis (SRH) and microneutralization (MN). Assays utilizing pseudotypes, chimeric viruses bearing influenza glycoproteins, have been shown to be highly efficient for the measurement of homosubtypic and heterosubtypic broadly-neutralizing antibodies, making them ideal serological tools for the study of cross-protective responses against multiple influenza subtypes with pandemic potential. In this review, we will analyze and compare literature involving the production of influenza pseudotypes with particular emphasis on their use in serum antibody neutralization assays. This will enable us to establish the parameters required for optimization and propose a consensus protocol to be employed for the further deployment of these assays in influenza vaccine immunogenicity studies.

Item Type: Article
Uncontrolled keywords: influenza, hemagglutinin, pseudotype, Neutralization assay, Universal vaccine, Lentiviral vectors, Retroviral vectors
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 26 Mar 2015 06:41 UTC
Last Modified: 18 Jan 2017 02:54 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/47786 (The current URI for this page, for reference purposes)
  • Depositors only (login required):

Downloads

Downloads per month over past year