Skip to main content

Ceramide triggers budding of exosome vesicles into multivesicular endosomes.

Trajkovic, Katarina, Hsu, Chieh, Chiantia, Salvatore, Rajendran, Lawrence, Wenzel, Dirk, Wieland, Felix, Schwille, Petra, Brügger, Britta, Simons, Mikael (2008) Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science (New York, N.Y.), 319 (5867). pp. 1244-1247. ISSN 1095-9203. (doi:10.1126/science.1153124) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://www.sciencemag.org/content/319/5867/1244

Abstract

Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation.

Item Type: Article
DOI/Identification number: 10.1126/science.1153124
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Chieh Hsu
Date Deposited: 16 Mar 2015 11:38 UTC
Last Modified: 29 May 2019 14:21 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/47684 (The current URI for this page, for reference purposes)
Hsu, Chieh: https://orcid.org/0000-0002-4780-1222
  • Depositors only (login required):