Hsu, Chieh, Morohashi, Yuichi, Yoshimura, Shin-Ichiro, Manrique-Hoyos, Natalia, Jung, Sangyong, Lauterbach, Marcel A, Bakhti, Mostafa, Grønborg, Mads, Möbius, Wiebke, Rhee, Jeongseop, and others. (2010) Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C. The Journal of cell biology, 189 (2). pp. 223-232. ISSN 1540-8140. (doi:10.1083/jcb.200911018) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:47683)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://dx.doi.org/10.1083/jcb.200911018 |
Abstract
Oligodendrocytes secrete vesicles into the extracellular space, where they might play a role in neuron-glia communication. These exosomes are small vesicles with a diameter of 50-100 nm that are formed within multivesicular bodies and are released after fusion with the plasma membrane. The intracellular pathways that generate exosomes are poorly defined. Because Rab family guanosine triphosphatases (GTPases) together with their regulators are important membrane trafficking organizers, we investigated which Rab GTPase-activating proteins interfere with exosome release. We find that TBC1D10A-C regulate exosome secretion in a catalytic activity-dependent manner. We show that Rab35 is the target of TBC1D10A-C and that the inhibition of Rab35 function leads to intracellular accumulation of endosomal vesicles and impairs exosome secretion. Rab35 localizes to the surface of oligodendroglia in a GTP-dependent manner, where it increases the density of vesicles, suggesting a function in docking or tethering. These findings provide a basis for understanding the biogenesis and function of exosomes in the central nervous system.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1083/jcb.200911018 |
| Subjects: |
Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Chieh Hsu |
| Date Deposited: | 16 Mar 2015 11:45 UTC |
| Last Modified: | 05 Nov 2024 10:31 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/47683 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Hsu, Chieh.
| Creator's ORCID: |
 https://orcid.org/0000-0002-4780-1222
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|---|---|
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