Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C.

Hsu, Chieh and Morohashi, Yuichi and Yoshimura, Shin-Ichiro and Manrique-Hoyos, Natalia and Jung, Sangyong and Lauterbach, Marcel A and Bakhti, Mostafa and Grønborg, Mads and Möbius, Wiebke and Rhee, Jeongseop and Barr, Francis A and Simons, Mikael (2010) Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C. The Journal of cell biology, 189 (2). pp. 223-232. ISSN 1540-8140. (doi:https://doi.org/10.1083/jcb.200911018) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1083/jcb.200911018

Abstract

Oligodendrocytes secrete vesicles into the extracellular space, where they might play a role in neuron-glia communication. These exosomes are small vesicles with a diameter of 50-100 nm that are formed within multivesicular bodies and are released after fusion with the plasma membrane. The intracellular pathways that generate exosomes are poorly defined. Because Rab family guanosine triphosphatases (GTPases) together with their regulators are important membrane trafficking organizers, we investigated which Rab GTPase-activating proteins interfere with exosome release. We find that TBC1D10A-C regulate exosome secretion in a catalytic activity-dependent manner. We show that Rab35 is the target of TBC1D10A-C and that the inhibition of Rab35 function leads to intracellular accumulation of endosomal vesicles and impairs exosome secretion. Rab35 localizes to the surface of oligodendroglia in a GTP-dependent manner, where it increases the density of vesicles, suggesting a function in docking or tethering. These findings provide a basis for understanding the biogenesis and function of exosomes in the central nervous system.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Chieh Hsu
Date Deposited: 16 Mar 2015 11:45 UTC
Last Modified: 17 Jun 2016 08:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/47683 (The current URI for this page, for reference purposes)
Hsu, Chieh: https://orcid.org/0000-0002-4780-1222
  • Depositors only (login required):