Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution.

Hsu, Chieh and Scherrer, Simone and Buetti-Dinh, Antoine and Ratna, Prasuna and Pizzolato, Julia and Jaquet, Vincent and Becskei, Attila (2012) Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution. Nature communications, 3 . Article 682. ISSN 2041-1723. (doi:https://doi.org/10.1038/ncomms1687) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1038/ncomms1687

Abstract

During evolution, genetic networks are rewired through strengthening or weakening their interactions to develop new regulatory schemes. In the galactose network, the GAL1/GAL3 paralogues and the GAL2 gene enhance their own expression mediated by the Gal4p transcriptional activator. The wiring strength in these feedback loops is set by the number of Gal4p binding sites. Here we show using synthetic circuits that multiplying the binding sites increases the expression of a gene under the direct control of an activator, but this enhancement is not fed back in the circuit. The feedback loops are rather activated by genes that have frequent stochastic bursts and fast RNA decay rates. In this way, rapid adaptation to galactose can be triggered even by weakly expressed genes. Our results indicate that nonlinear stochastic transcriptional responses enable feedback loops to function autonomously, or contrary to what is dictated by the strength of interactions enclosing the circuit.

Item Type: Article
Uncontrolled keywords: Biological sciences Biotechnology Evolution Microbiology
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Chieh Hsu
Date Deposited: 16 Mar 2015 13:12 UTC
Last Modified: 17 Jun 2016 08:06 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/47681 (The current URI for this page, for reference purposes)
Hsu, Chieh: https://orcid.org/0000-0002-4780-1222
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