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The application of pseudotypes to Influenza pandemic preparedness

Ferrara, Francesca, Molesti, Eleonora, Temperton, Nigel J. (2015) The application of pseudotypes to Influenza pandemic preparedness. Future Virology, 10 (6). pp. 731-749. (doi:10.2217/fvl.15.36) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.2217/fvl.15.36

Abstract

Human and animal populations are constantly exposed to multiple influenza strains due to zoonotic spillover and rapid viral evolution driven by intrinsic error­prone replication and immunological pressure. In this context, antibody responses directed against the haemagglutinin protein on the surface of the virus are of importance since they have been shown to correlate with protective immunity. Serological techniques, detecting these responses, play a critical role in influenza pandemic preparedness in particular with regards to the measurement of vaccine immunogenicity. As the recent human pandemics (H1N1) and avian influenza outbreaks (H5 and H7) have demonstrated, there is an urgent need to be better prepared to assess the contribution of the antibody response to protection against newly emerged viruses and to evaluate the extent of pre-existing hetero-subtypic immunity in populations. This review compares pseudotype-based assays with wildtype and VLP virus assays and discusses their place in the pandemic preparedness against the influenza virus. It additionally addresses the state of the art developments of pseudotype-based assays (chimeric haemagglutinins, multiplex and post-attachment) including the development and future deployment of assay kits and approaches towards standardization to both pre-clinical and clinical endpoints. Progress towards the development of an influenza pseudotype library for the purposes of pandemic preparedness is also outlined and discussed.

Item Type: Article
DOI/Identification number: 10.2217/fvl.15.36
Uncontrolled keywords: Pseudotypes, Pandemic preparedness, Neutralization assays, Universal vaccines, Pseudotype library
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 07 Mar 2015 08:12 UTC
Last Modified: 29 May 2019 14:19 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/47584 (The current URI for this page, for reference purposes)
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