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A Rapid Two-Step Procedure for the Purification of Human Peripheral Blood Basophils to Near Homogeneity

Gibbs, Bernhard F, Papenfuss, K., Falcone, Franco H. (2008) A Rapid Two-Step Procedure for the Purification of Human Peripheral Blood Basophils to Near Homogeneity. Clinical Experimental Allergy, 38 (3). pp. 480-485. ISSN 0954-7894. E-ISSN 1365-2222. (doi:10.1111/j.1365-2222.2007.02919.x) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:4754)

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http://dx.doi.org/10.1111/j.1365-2222.2007.02919.x

Abstract

Background Basophils are increasingly utilized as indicators of allergic inflammation and as primary allergic effector cells to study signalling pathways. However, until the present, their enrichment has been time consuming, costly and limited to relatively few specialized laboratories.

Conclusion The rapidity, simplicity and reproducibility of this method will facilitate the employment of basophils in high-output ex vivo studies.

Item Type: Article
DOI/Identification number: 10.1111/j.1365-2222.2007.02919.x
Uncontrolled keywords: basophils; CD203c; CD63; histamine; IgE; IL-4; purification techniques
Subjects: R Medicine
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Bernhard F. Gibbs
Date Deposited: 04 Mar 2009 13:45 UTC
Last Modified: 28 May 2019 13:40 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/4754 (The current URI for this page, for reference purposes)
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