Bat and pig Interferon-Induced Transmembrane Protein 3 restrict cell entry by influenza virus and lyssaviruses

Interferon-induced transmembrane protein 3 (IFITM3) is a restriction factor which

pathways. In humans and mice, IFITM3 limits influenza-induced morbidity and

function as antiviral restriction factors in mammalian species other than humans and

(Sus scrofa domesticus) were identified using rapid amplification of cDNA ends. Amino

bat orthologues. Ectopically-expressed pig and microbat IFITM3 co-localised with

trafficked from the plasma membrane into endosomes following live cell staining. Pig

and lyssavirus G proteins. Expression of pig or microbat IFITM3 in A549 cells reduced

IFITM3 in pig NPTr cells and primary microbat cells enhanced virus replication,

levels. In sum, we show that IFITMs function as potent broad-spectrum antiviral

viruses.