Skip to main content
Kent Academic Repository

Expression and localization of creatine kinase in the preimplantation embryo.

Forsey, Katherine E., Ellis, Peter J.I., Sargent, Carole A., Sturmey, Roger G., Leese, Henry J. (2013) Expression and localization of creatine kinase in the preimplantation embryo. Molecular reproduction and development, 80 (3). pp. 185-92. ISSN 1098-2795. (doi:10.1002/mrd.22146) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:46546)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1002/mrd.22146

Abstract

Creatine Kinase (CK) catalyses the "creatine shuttle," the reversible conversion of creatine phosphate to creatine with the liberation of ATP. This article examines the potential role of the creatine shuttle in the provision of ATP during mouse preimplantation embryo development. Using quantitative PCR, transcripts of four subunit isoforms of CK--CKM, CKB, CKMT1, and CKMT2--were detectable at all developmental stages, from the presumptive zygote to late blastocyst, but there was no obvious pattern in gene expression. By contrast, total CK biochemical activity, measured by a novel method, was relatively constant from the 2- to 8-cell stage, before exhibiting a significant decrease in activity at the blastocyst stage. Immunocytochemical studies revealed a marked association of CKB with the mitotic spindle in 2- and 4-cell mouse embryos, consistent with the proposition that the creatine shuttle plays a key role in local delivery of ATP during cytokinesis. Endogenous creatine was detected in the blastocyst at a level of 0.53 pmol/embryo. In conclusion, we believe that creatine phosphate can now be added to the list of potential sources of ATP during preimplantation development.

Item Type: Article
DOI/Identification number: 10.1002/mrd.22146
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Peter Ellis
Date Deposited: 19 Jan 2015 17:22 UTC
Last Modified: 09 Mar 2023 11:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/46546 (The current URI for this page, for reference purposes)

University of Kent Author Information

  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.