Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection

Ahmed, Muhammad S and Jacques, Laura C and Mahallawi, Waleed H and Ferrara, Francesca and Temperton, Nigel J. and Upile, Nav and Vaughan, Casey and Sharma, Ravi and Beer, Helen and Hoschler, Katja and McNamara, Paul S and Zhang, Qibo (2014) Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection. Antiviral Research, 114 . pp. 106-112. ISSN 0166-3542. (doi:https://doi.org/10.1016/j.antiviral.2014.12.008) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

2009 H1N1 pandemic influenza (A(H1N1)pdm09) virus infected large numbers of people worldwide. Recent studies suggest infection with A(H1N1)pdm09 virus elicited cross-reactive anti-hemagglutinin (HA) memory B cell response to conserved regions of HA. However, the breadth and magnitude of cross-reactive immunity in children and adults following A(H1N1)pdm09 infection are unknown. Methods: We investigated serum anti-HA immunity to a number of group-1 and -2 viruses in children and adults using hemagglutination inhibition (HAI), enzyme-linked immunosorbent assay and virus neutralization assay. Results: Applying hemagglutination inhibition (HAI) titres?40 against A(H1N1)pdm09 as threshold of sero-positivity, we observed significantly higher levels of anti-HA antibodies to a number of virus subtypes, including those neutralizing H5N1, in subjects with HAI titre?40 than those with HAI<40. Adults demonstrated broader and stronger cross-reactive anti-HA antibodies than children, including cross-reactive anti-HA1 and -HA2 antibodies. By comparison, individuals with serologic evidence of recent exposure to seasonal H1N1 or H3N2 did not show such broad cross-reactive immunity. Conclusion: Our results suggest individuals exposed to A(H1N1)pdm09 virus developed a broad and age-associated cross-reactive anti-HA immunity which may have important implications for future vaccination strategies to enable protection against a broader range of influenza viruses.

Item Type: Article
Uncontrolled keywords: Influenza virus; Pandemic H1N1 infection; Serum antibody; Cross-reactive immunity; Anti-hemagglutinin antibody; Virus neutralization
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 14 Dec 2014 21:20 UTC
Last Modified: 08 May 2018 08:48 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/46067 (The current URI for this page, for reference purposes)
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