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Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection

Ahmed, Muhammad S, Jacques, Laura C, Mahallawi, Waleed H, Ferrara, Francesca, Temperton, Nigel J., Upile, Nav, Vaughan, Casey, Sharma, Ravi, Beer, Helen, Hoschler, Katja, and others. (2014) Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection. Antiviral Research, 114 . pp. 106-112. ISSN 0166-3542. (doi:10.1016/j.antiviral.2014.12.008) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:46067)

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2009 H1N1 pandemic influenza (A(H1N1)pdm09) virus infected large numbers of people worldwide. Recent studies suggest infection with A(H1N1)pdm09 virus elicited cross-reactive anti-hemagglutinin (HA) memory B cell response to conserved regions of HA. However, the breadth and magnitude of cross-reactive immunity in children and adults following A(H1N1)pdm09 infection are unknown. Methods: We investigated serum anti-HA immunity to a number of group-1 and -2 viruses in children and adults using hemagglutination inhibition (HAI), enzyme-linked immunosorbent assay and virus neutralization assay. Results: Applying hemagglutination inhibition (HAI) titres?40 against A(H1N1)pdm09 as threshold of sero-positivity, we observed significantly higher levels of anti-HA antibodies to a number of virus subtypes, including those neutralizing H5N1, in subjects with HAI titre?40 than those with HAI<40. Adults demonstrated broader and stronger cross-reactive anti-HA antibodies than children, including cross-reactive anti-HA1 and -HA2 antibodies. By comparison, individuals with serologic evidence of recent exposure to seasonal H1N1 or H3N2 did not show such broad cross-reactive immunity. Conclusion: Our results suggest individuals exposed to A(H1N1)pdm09 virus developed a broad and age-associated cross-reactive anti-HA immunity which may have important implications for future vaccination strategies to enable protection against a broader range of influenza viruses.

Item Type: Article
DOI/Identification number: 10.1016/j.antiviral.2014.12.008
Uncontrolled keywords: Influenza virus; Pandemic H1N1 infection; Serum antibody; Cross-reactive immunity; Anti-hemagglutinin antibody; Virus neutralization
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 14 Dec 2014 21:20 UTC
Last Modified: 17 Aug 2022 10:58 UTC
Resource URI: (The current URI for this page, for reference purposes)
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