Construction and properties of a turkey herpesvirus recombinant expressing the Marek's disease virus homologue of glycoprotein-B of herpes-simplex virus.

Ross, L.J.N. and Binns, M.M. and Tyers, P. and Pastorek, J. and Zelnik, V. and Scott, Simon D. (1993) Construction and properties of a turkey herpesvirus recombinant expressing the Marek's disease virus homologue of glycoprotein-B of herpes-simplex virus. Journal of General Virology, 74 (3). pp. 371-377. ISSN 0022-1317. (doi:https://doi.org/10.1099/0022-1317-74-3-371) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1099/0022-1317-74-3-371

Abstract

A herpesvirus of turkeys (HVT) recombinant containing a 3.9 kbp fragment of Marek's disease virus (MDV) DNA encoding MDV glycoprotein B (gB), stably integrated into the thymidine kinase (TK) gene of HVT, has been constructed. The replication of the recombinant in chick embryo fibroblasts (CEF) was comparable to that of wild-type HVT. The recombinant expressed authentic MDV gB and its processed forms (110K, 65K and 48K) in CEF as shown by immunoblotting using an MDV-specific anti-peptide serum. Northern blot analysis showed that MDV gB mRNA was transcribed from MDV promoter sequences flanking the MDV gB open reading frame and also from the HVT TK promoter. However, the level of replication of the recombinant in vivo appeared to be lower than wild-type HVT as shown by the titres of HVT antibodies, determined by ELISA. Pathogenicity tests showed that the recombinant was safe and did not cause microscopic or gross Marek's disease lesions or other abnormalities. The results suggest that HVT has potential as a vector for recombinant vaccines.

Item Type: Article
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Simon Scott
Date Deposited: 03 Dec 2017 14:22 UTC
Last Modified: 05 Dec 2017 10:12 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45902 (The current URI for this page, for reference purposes)
Scott, Simon D.: https://orcid.org/0000-0002-8290-0461
  • Depositors only (login required):