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A novel enzyme/prodrug system for hypoxia- and radiation-regulated suicide gene therapy

Greco, O., Scott, Simon D., Tozer, G.M., Marples, B., Joiner, M.C., Dachs, G.U. (2001) A novel enzyme/prodrug system for hypoxia- and radiation-regulated suicide gene therapy. In: Cancer Gene Therapy. 8 (11). Springer-Nature (doi:10.1038/sj.cgt.7700412) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:45888)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1038/sj.cgt.7700412

Abstract

We have previously proposed the plant enzyme horseradish peroxidase

novel enzyme/ prodrug combination for suicide gene therapy ( Greco et al.

systems ( e.g. HSV TK/ GCV ), HRP/ IAA has the advantage of inducing

also in the tumour conditions of anoxia and hypoxia ( 0.1% O2 ). Here we

tumoricidal action of ionising radiation. Incubation with IAA prior to or postX - rays induced an increase in sensitivity of HRP - expressing cells of 2.5 –

itself or exposure of HRP ?cells to IAA did not affect the response to

solid tumours, the HRP gene was placed under the control of hypoxia - or

( HREs ) from the PGK - 1 or the EPO genes were inserted upstream of

EPO and the PGK - 1 HREs induced a 30 – 40 - fold and a 5 – 6 - fold increase

conferred hypoxia - selective sensitivity to IAA and analogues. HRP

CArG elements (Marples et al. Gene Therapy. 7: 511, 2000 ). After 5 Gy X -

detected.

Item Type: Conference or workshop item (Proceeding)
DOI/Identification number: 10.1038/sj.cgt.7700412
Subjects: R Medicine
R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Simon Scott
Date Deposited: 03 Dec 2017 15:26 UTC
Last Modified: 29 May 2019 13:52 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45888 (The current URI for this page, for reference purposes)
Scott, Simon D.: https://orcid.org/0000-0002-8290-0461
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