Gene silencing approaches in mast cells and primary human basophils.

Sumbayev, Vadim V. and Gibbs, Bernhard F. (2014) Gene silencing approaches in mast cells and primary human basophils. In: Gibbs, Bernhard F. and Sumbayev, Vadim V., eds. Basophils and Mast Cless. Methods in Molecular Biology . Springer New York, pp. 185-191. ISBN 978-1-4939-1172-1. E-ISBN 978-1-4939-1173-8. (doi:https://doi.org/10.1007/978-1-4939-1173-8_14) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

The ability to silence gene expression is an invaluable tool for elucidating the importance of intracellular signaling proteins which contribute to the effector functions of mast cells and basophils. However, primary mast cells and their terminally differentiated blood counterpart, namely basophils, pose a difficult challenge for gene silencing approaches given not only their state of maturation and difficulty to transfect, but also because their functions are readily altered by cell-handling conditions. Here, we describe a method using lipofection which has been successfully employed to silence gene expression using siRNA in human LAD2 mast cells as well as primary human basophils.

Item Type: Book section
Projects: [UNSPECIFIED] Mast Cells and Basophils - Targets for Innovative Therapies
Subjects: Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Bernhard F. Gibbs
Date Deposited: 03 Dec 2014 13:44 UTC
Last Modified: 03 Dec 2014 13:44 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45735 (The current URI for this page, for reference purposes)
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