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Basophil stimulation and signaling pathways

Knol, Edward F. and Gibbs, Bernhard F. (2014) Basophil stimulation and signaling pathways. In: Gibbs, Bernhard F and Falcone, Franco H., eds. Basophils and Mast Cells. Methods in Molecular Biology, 1192 . Springer New York, pp. 193-203. ISBN 978-1-4939-1172-1. E-ISBN 978-1-4939-1173-8. (doi:10.1007/978-1-4939-1173-8_15) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
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Abstract

Despite growing use of flow cytometry to analyze the functional characteristics of primary basophils the intracellular signaling cascades that control their ability to elaborate various inflammatory mediators and cytokines remain comparatively obscure. Additionally, some studies require the analysis of pro-allergic and inflammatory mediators, such as histamine, LTC4, and various basophil-derived cytokines (e.g., IL-4 and IL-13). Elucidation of intracellular signaling proteins by Western blotting, cytosolic free calcium concentration by spectrofluorophotometry, and detection of mediator releases, as well as analysis of gene expressions by RT-PCR, generally require relatively large numbers of purified basophils. In selected assays, flow cytometry can enable the analysis of relatively low cell numbers and purity for the expression of intracellular signaling proteins or measurement of cytosolic free calcium concentrations by basophil-specific gating strategies. Unfortunately, many aspects of signal transduction relevant to human basophils cannot be readily extrapolated from the use of basophil or mast cell lines. This chapter therefore focuses on how to employ primary human basophils for studying mediator releases and signaling characteristics.

Item Type: Book section
DOI/Identification number: 10.1007/978-1-4939-1173-8_15
Projects: [UNSPECIFIED] Mast Cells and Basophils - Targets for Innovative Therapies
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Bernhard F. Gibbs
Date Deposited: 03 Dec 2014 13:16 UTC
Last Modified: 29 May 2019 13:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45732 (The current URI for this page, for reference purposes)
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