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Microscopic Analysis of the Interaction of Gold Nanoparticles with Cells of the Innate Immune System

Pascual García, César, Sumbayev, Vadim V., Gilliland, Douglas, Yasinska, Inna M., Gibbs, Bernhard F., Mehn, Dora, Calzolai, Luigi, Rossi, François (2013) Microscopic Analysis of the Interaction of Gold Nanoparticles with Cells of the Innate Immune System. Scientific Reports, 3 . p. 1326. ISSN 2045-2322. (doi:10.1038/srep01326) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1038/srep01326

Abstract

Gold nanoparticles (AuNPs) can interfere with some of the biochemical processes of macrophage cells but the mechanisms of action of these potentially medically-relevant effects are still unclear. Here we study the fate of AuNPs interacting with cells derived from the innate immune system. To visualize AuNPs with nanometer resolution without losing sight of the whole morphology of cells we developed a convenient approach that uses electron and ion microscopy techniques. The inspection using an ion beam to selectively cut where the AuNPs were found, allows for determining their intracellular localizations. We studied the cellular uptake of AuNPs, with or without exposure of the cells to Latrunculin-A, a phagocytosis inhibitor. Results indicate a size dependence of the internalization mechanisms for THP-1 cells. The internalization of larger AuNPs was blocked in the presence of Latrunculin-A, although they could attach to the membrane. Smaller AuNPs though were not blocked by actin depending processes.

Item Type: Article
DOI/Identification number: 10.1038/srep01326
Subjects: Q Science > QC Physics > QC176.8.N35 Nanoscience, nanotechnology
Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Bernhard F. Gibbs
Date Deposited: 03 Dec 2014 10:51 UTC
Last Modified: 29 May 2019 13:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45719 (The current URI for this page, for reference purposes)
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