Skip to main content

High efficiency amine functionalization of cycloolefin polymer surfaces for biodiagnostics

Gandhiraman, R.P., Volcke, C., Gubala, V., Doyle, C., Basabe-Desmonts, L., Dotzler, C., Toney, M.F., Iacono, M., Nooney, R.I., Daniels, S., and others. (2010) High efficiency amine functionalization of cycloolefin polymer surfaces for biodiagnostics. Journal of Materials Chemistry, 20 (20). pp. 4116-4127. ISSN 0959-9428. (doi:10.1039/b925737c) (KAR id:45240)


Point-of-care (POC) diagnostics implementing microfluidic technology on single use disposable plastic chips has potential applications in personalized medicine, clinical diagnostics and global health. However, the challenges in commercializing POC devices must be addressed. Immobilization of biomolecules to plastic chips through appropriate surface functionalization is a key issue for the fabrication of new generation biomedical diagnostic devices. The most important requirements for a practicable surface functionalization process are speed, control and reliability. Plasma-based methods can meet these criteria. A single step, solventless, ecofriendly and high throughput nature of plasma processing makes them highly attractive. Here we demonstrate the efficient surface functionalization of a next-generation biosensor material, a chemically inert cycloolefin polymer (COP). The plasma formation of a surface-bound aminated siloxane network from mixed aminopropyltriethoxysilane and ethylenediamine precursors allowed us to form a well-adherent film with an exceptionally high degree of amine functionalization. We deduce that the siloxane was the critical component for radical insertion into the COP and for building a stable network to support the reactive amine functionalities. We present a full physical and chemical characterization of the films, including a detailed study of their swelling in water, using an array of surface analytical techniques: X-ray photoelectron spectroscopy, X-ray reflectivity, reflection infra-red spectroscopy, atomic force microscopy (AFM) and fluorophore binding reactions. We demonstrate an original approach for qualitatively analyzing the distribution of amine functionalities by counting surface-bound functionalized silica nanoparticles in the AFM. The relative contributions from covalent (specific) and non-covalent (non-specific) reaction chemistry assessed using 3�-fluorescein-labeled ssDNA attachment showed that the non-specific binding could be reduced significantly according to the particular feed gas mixture used to prepare the coating. A reaction mechanism has been proposed for the deposition of amine functionalities on COP plastic and also for enhancing the amine functionalities that affect the non-specific binding significantly. © 2010 The Royal Society of Chemistry.

Item Type: Article
DOI/Identification number: 10.1039/b925737c
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Mater. Chem. [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute (BDI), Dublin City University, Collins Avenue, Glasnevin Dublin 9, Ireland [Field not mapped to EPrints] AD - Research Centre in Physics of Matter and Radiation (PMR), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium [Field not mapped to EPrints] AD - Research Centre for Surface and Materials Science, Department of Chemical and Materials Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand [Field not mapped to EPrints] AD - Industrial Research Limited, Box 31-310, 69 Gracefield Road P.O., Lower Hutt 5040, New Zealand [Field not mapped to EPrints] AD - Stanford Synchrotron Radiation Lightsource, 2575 Sand Hill Road, Menlo Park, CA 94025, United States [Field not mapped to EPrints] AD - National Centre for Plasma Science and Technology, Dublin City University, Collins Avenue, Glasnevin Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Adherent films, AFM, Amine functionality, Amine functionalization, Aminopropyltriethoxysilane, Analytical techniques, Binding reactions, Bio medical diagnostics, Biodiagnostics, Chemical characterization, Clinical diagnostics, Critical component, Cyclo-olefin polymers, Eco-friendly, Efficient surface, Ethylene diamine, Feed gas, Functionalized silica, Global health, High efficiency, High throughput, Key issues, Microfluidic technologies, Non-specific binding, Personalized medicines, Plasma formations, Plasma processing, Plastic chips, Point of care, Potential applications, Reaction chemistry, Reaction mechanism, Relative contribution, Single-step, Solventless, Stable network, Surface Functionalization, X ray reflectivity, Atomic force microscopy, Atomic spectroscopy, Binding sites, Biological materials, Biosensors, Organic compounds, Plastics, Scanning electron microscopy, Silica, Surface chemistry, Surfaces, X ray diffraction, X ray photoelectron spectroscopy, Functional polymers
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 20:57 UTC
Last Modified: 16 Nov 2021 10:18 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.